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Capillary Electrophoresis of Proteins: Chromatographic Science Series

Editat de Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu
en Limba Engleză Hardback – 20 oct 1998
"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."
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Specificații

ISBN-13: 9780824702052
ISBN-10: 0824702050
Pagini: 300
Ilustrații: illustrations
Dimensiuni: 152 x 229 x 20 mm
Greutate: 0.72 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Chromatographic Science Series


Public țintă

Professional

Cuprins

Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.

Recenzii

". . .well written and referenced. It is one of the most comprehensive texts currently available on the application of capillary electrophoresis to protein analysis. . ..a useful source of information for separation scientists and postgraduate students wishing to obtain an overall review of protein capillary electrophoresis separations."
---European Journal of Pharmaceutics and Biopharmaceutics

Notă biografică

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu

Descriere

"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."