Cilia: Motors and Regulation: Methods in Cell Biology, cartea 92
Stephen M. King, Gregory J Pazouren Limba Engleză Hardback – 4 dec 2009
- Covers protocols for cilia and flagella across systems and species
- Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time
- Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
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Specificații
ISBN-13: 9780123749741
ISBN-10: 0123749743
Pagini: 400
Dimensiuni: 191 x 235 x 18 mm
Greutate: 0.66 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology
Locul publicării:United Kingdom
ISBN-10: 0123749743
Pagini: 400
Dimensiuni: 191 x 235 x 18 mm
Greutate: 0.66 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology
Locul publicării:United Kingdom
Public țintă
Researchers and students in cell, molecular and developmental biology.Cuprins
MCB, 92
1. Bioinformatic Approaches to Dynein Heavy Chain Classification
Toshiki Yagi
2. Identification and Characterization of Dynein Genes in Tetrahymena
David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,
Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson, Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva, Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva, Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson, Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai
3. Purification of Axonemal Dyneins and Dynein-Associated Components
from Chlamydomonas
Stephen M. King
4. Purification of Dyneins from Sperm Flagella
Kazuo Inaba and Katsutoshi Mizuno
5. Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression System
Takahide Kon, Tomohiro Shima, and Kazuo Sutoh
6. Biophysical Measurements on Axonemal Dyneins
Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa
7. Protein Electroporation into Chlamydomonas for Mutant Rescue
Masahito Hayashi and Ritsu Kamiya
8. Analysis of the Role of Nucleotides in Axonemal Dynein Function
Chikako Shingyoji
9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas
Flagella by Axonemal Kinases and Phosphatases
Candice A. Elam, Winfield S. Sale, and Maureen Wirschell
10. Analysis of Redox-Sensitive Dynein Components
Ken-ichi Wakabayashi
11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins
Christen DiPetrillo and Elizabeth Smith
12. Isolation and Analysis of Radial Spoke Proteins
Pooja Kelekar, Mei Wei, and Pinfen Yang
13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii
David R. Mitchell and Brandon Smith
1. Bioinformatic Approaches to Dynein Heavy Chain Classification
Toshiki Yagi
2. Identification and Characterization of Dynein Genes in Tetrahymena
David E. Wilkes, Nicole Bennardo, Clarence W.C. Chan, Yu-Loung Chang,
Elizabeth O. Corpuz, Jennifer DuMond, Jordan A. Eboreime, Julianna Erickson, Jonathan Hetzel, Erin E. Heyer, Mark J. Hubenschmidt, Ekaterina Kniazeva, Hallie Kuhn, Michelle Lum, Andrea Sand, Alicia Schep, Oksana Sergeeva, Natt Supab, Caroline R. Townsend, Liesl Van Ryswyk, Hadley E. Watson, Alice E. Wiedeman, Vidyalakshmi Rajagopalan, and David J. Asai
3. Purification of Axonemal Dyneins and Dynein-Associated Components
from Chlamydomonas
Stephen M. King
4. Purification of Dyneins from Sperm Flagella
Kazuo Inaba and Katsutoshi Mizuno
5. Protein Engineering Approaches to Study the Dynein Mechanism using a Dictyostelium Expression System
Takahide Kon, Tomohiro Shima, and Kazuo Sutoh
6. Biophysical Measurements on Axonemal Dyneins
Hiroaki Kojima, Shiori Toba, Hitoshi Sakakibara, and Kazuhiro Oiwa
7. Protein Electroporation into Chlamydomonas for Mutant Rescue
Masahito Hayashi and Ritsu Kamiya
8. Analysis of the Role of Nucleotides in Axonemal Dynein Function
Chikako Shingyoji
9. The Regulation of Dynein-Driven Microtubule Sliding in Chlamydomonas
Flagella by Axonemal Kinases and Phosphatases
Candice A. Elam, Winfield S. Sale, and Maureen Wirschell
10. Analysis of Redox-Sensitive Dynein Components
Ken-ichi Wakabayashi
11. Calcium Regulation of Ciliary Motility: Analysis of Axonemal Calcium-Binding Proteins
Christen DiPetrillo and Elizabeth Smith
12. Isolation and Analysis of Radial Spoke Proteins
Pooja Kelekar, Mei Wei, and Pinfen Yang
13. Analysis of the Central Pair Microtubule Complex in Chlamydomonas reinhardtii
David R. Mitchell and Brandon Smith