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DNA Repair Enzymes: Cell, Molecular, and Chemical Biology de Brandt Eichman

DNA Repair Enzymes: Cell, Molecular, and Chemical Biology: Methods in Enzymology, cartea 591

Brandt Eichman
en Limba Engleză Hardback – 20 iun 2017
DNA Repair Enzymes, Part A, Volume 591 is the latest volume in the Methods in Enzymology series and the first part of a thematic that focuses on DNA repair enzymes. Topics in this new release include chapters on the Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells, the Proteomic Analyses of the Eukaryotic Replication Machinery, DNA Fiber Analysis: Mind the Gap!, Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells, Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner, Base Excision Repair Variants in Cancer, and Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli.


  • Includes contributions from leading authorities working in enzymology
  • Focuses on DNA repair enzymes
  • Informs and updates on all the latest developments in the field of enzymology
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Specificații

ISBN-13: 9780128118467
ISBN-10: 0128118466
Pagini: 478
Dimensiuni: 152 x 229 x 31 mm
Greutate: 0.93 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Scientists interested in modern nucleic acid biology, as well as to scientists outside the field interested in learning how the field has expanded to encompass a broad range of topics, pathways, and methods

Cuprins

1. Optimization of Native and Formaldehyde iPOND Techniques for Use in Suspension Cells
Nathaniel E. Wiest and Alan E. Tomkinson
2. Proteomic Analyses of the Eukaryotic Replication Machinery
David Cortez
3. DNA Fiber Analysis: Mind the Gap!
Annabel Quinet, Denisse Carvajal-Maldonado, Delphine Lemacon and Alessandro Vindigni
4. Comet-FISH for Ultrasensitive Strand-Specific Detection of DNA Damage in Single Cells
Manas Mondal and Jia Guo
5. Examining DNA Double-Strand Break Repair in a Cell Cycle-Dependent Manner
Janapriya Saha, Shih-Ya Wang and Anthony J. Davis
6. Base Excision Repair Variants in Cancer
Carolyn G. Marsden, Julie A. Dragon, Susan S. Wallace and Joann B. Sweasy
7. Fluorescence-Based Reporters for Detection of Mutagenesis in E. coli
Melissa Standley, Jennifer Allen, Layla Cervantes, Joshua Lilly and Manel Camps
8. Sequencing DNA for the Oxidatively Modified Base 8-Oxo-7,8-Dihydroguanine
Aaron M. Fleming, Yun Ding and Cynthia J. Burrows
9. Xenopus laevis as Model System to Study DNA Damage Response and Replication Fork Stability
Vincenzo Sannino, Federica Pezzimenti, Stefania Bertora and Vincenzo Costanzo
10. Ensemble and Single-Molecule Analysis of NHEJ in Frog Egg Extracts
Thomas Graham, Johannes C. Walter and Joseph J. Loparo
11. Analysis of Structure-Selective Endonuclease Activities From Yeast and Human Extracts
Joao Matos and Stephen C. West
12. Strategies and Methods of Transcription-Coupled Repair Studies In Vitro and In Vivo 
Vitaly Epshtein, Venu Kamarthapu and Evgeny Nudler
13. Reconstituted System for the Examination of Repair DNA Synthesis in Homologous Recombination
Youngho Kwon, James M. Daley and Patrick Sung
14. Current and Emerging Assays for Studying the Primer Synthesis Activities of DNA Primases
Thomas A. Guilliam and Aidan J. Doherty
15. Electrical Probes of DNA-Binding Proteins
Jacqueline K. Barton, Phillip L. Bartels, Yingxin Deng and Elizabeth O’Brien
16. Preparation of Stable Nitrogen Mustard DNA Interstrand Cross-Link Analogs for Biochemical and Cell Biological Studies
Alejandra Castaño, Upasana Roy and Orlando D. Schärer