Enzymes of Epigenetics: Methods in Enzymology, cartea 573
Ronen Marmorsteinen Limba Engleză Hardback – 13 iul 2016
This volume covers research methods that are employed to study epigenetic regulation and includes structural, biochemical, molecular, biological, cellular, computational, and systems approaches.
Topics include chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators, and biological connections
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Contains two new volumes covering research methods in enzymes of epigenetics
- Covers topics such as chromatin structure and histones, posttranslational histone modification enzymes and complexes, histone modification binders, DNA modifications and nucleic acid regulators, epigenetic technologies and small molecule epigenetic regulators and biological connections
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Specificații
ISBN-13: 9780128053652
ISBN-10: 0128053658
Pagini: 528
Dimensiuni: 152 x 229 x 33 mm
Greutate: 1.02 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
ISBN-10: 0128053658
Pagini: 528
Dimensiuni: 152 x 229 x 33 mm
Greutate: 1.02 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
Public țintă
Scientists who would like to move into the area of epigenetics research or to incorporate epigenetic studies into their own research programCuprins
Chromatin Structure and Histones 1. In Vitro Chromatin Assembly - Strategies and Quality Control Uma Muthurajan, Francesca Mattiroli, Serge Bergeron, Keda Zhou, Yajie Gu, Srinivas Chakravarthy, Pamela Dyer, Thomas Irving and Karolin Luger 2. Preparation, Crystallization and Structure Determination of Chromatin Enzyme/Nucleosome Complexes Robert K. McGinty, Ravindra D. Makde and Song Tan 3. Preparation of Recombinant Centromeric Nucleosomes and Formation of Complexes with Non-Histone Centromere Proteins Nikolina Sekulic and Ben E. Black 4. Functional Characterization of Histone Chaperones using SNAP-Tag-Based Imaging to Assess De Novo Histone Deposition Camille Clément, Isabelle Vassias, Dominique Ray-Gallet and Geneviève Almouzni 5. Analysis of Nucleosome Sliding by ATP-Dependent Chromatin Remodelling Enzymes Coral Y. Zhou and Geeta J. Narlikar
Posttranslational Histone Modification Enzymes and Complexes 6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening Cheryl E. McCullough and Ronen Marmorstein 7. Preparation and Biochemical Analysis of Classical Histone Deacetylases Alejandro Villagra, Eva Sahakian and Edward Seto 8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases Benjamin Suenkel and Clemens Steegborn 9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone Methyltransferases Elisa Bergamin and Jean Francois Couture 10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase Benoit Laurent and Yang Shi 11. LSD1 Histone Demethylase Assays and Inhibition Dawn Hayward and Philip A. Cole 12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases Swathi Krishnan and Raymond C. Trievel 13. Preparation and Analysis of Native Chromatin Modifying Complexes Yannick Doyon and Jacques Côté
Histone Modification Binders (Readers) 14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding Domain Chunyan Ren, Lei Zeng and Ming-Ming Zhou 15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine Readers Catherine A. Musselman and Tatiana G. Kutateladze
DNA Modifications and Nucleic Acid Regulators 16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli 17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng 18. Crystallographic Studies of Telomerase Hunter Hoffman and Emmanuel Skordalakes 19. Detection and Analysis of Long Non-Coding RNAs Fan Lai, Ezra Blumenthal and Ramin Shiekhattar 20. Identifying Centromeric RNAs Involved in Histone Dynamics in Vivo Delphine Quénet, David Sturgill and Yamini Dalal
Posttranslational Histone Modification Enzymes and Complexes 6. In Vitro Activity Assays for Myst Histone Acetyltransferases and Adaptation for High-Throughput Inhibitor Screening Cheryl E. McCullough and Ronen Marmorstein 7. Preparation and Biochemical Analysis of Classical Histone Deacetylases Alejandro Villagra, Eva Sahakian and Edward Seto 8. Recombinant Preparation, Biochemical Analysis, and Structure Determination of Sirtuin Family Histone/Protein Deacylases Benjamin Suenkel and Clemens Steegborn 9. Preparation, Biochemical Analysis and Structure Determination of SET Domain Histone Methyltransferases Elisa Bergamin and Jean Francois Couture 10. Expression, Purification and Biochemical Analysis of the LSD1/KDM1A Histone Demethylase Benoit Laurent and Yang Shi 11. LSD1 Histone Demethylase Assays and Inhibition Dawn Hayward and Philip A. Cole 12. Purification, Biochemical Analysis, and Structure Determination of JmjC Lysine Demethylases Swathi Krishnan and Raymond C. Trievel 13. Preparation and Analysis of Native Chromatin Modifying Complexes Yannick Doyon and Jacques Côté
Histone Modification Binders (Readers) 14. Preparation, Biochemical Analysis and Structure Determination of the Bromodomain, an Acetyl-lysine Binding Domain Chunyan Ren, Lei Zeng and Ming-Ming Zhou 15. Preparation, Biochemical Analysis and Structure Determination of Methyllysine Readers Catherine A. Musselman and Tatiana G. Kutateladze
DNA Modifications and Nucleic Acid Regulators 16. Quantification of Oxidized 5-Methylcytosine Bases and TET Enzyme Activity Monica Yun Liu, Jamie E. DeNizio and Rahul M. Kohli 17. Characterization of how DNA Modifications Affect DNA Binding by C2H2 Zinc Finger Proteins Anamika Patel, Hideharu Hashimoto, Xing Zhang and Xiaodong Cheng 18. Crystallographic Studies of Telomerase Hunter Hoffman and Emmanuel Skordalakes 19. Detection and Analysis of Long Non-Coding RNAs Fan Lai, Ezra Blumenthal and Ramin Shiekhattar 20. Identifying Centromeric RNAs Involved in Histone Dynamics in Vivo Delphine Quénet, David Sturgill and Yamini Dalal
Recenzii
Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry "The work most often consulted in the lab." --Enzymologia "The Methods in Enzymology series represents the gold-standard." --Neuroscience