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Flow Cytometry Data Analysis: Basic Concepts and Statistics

Autor James V. Watson
en Limba Engleză Paperback – 14 sep 2005
Flow cytometry is now well established in research laboratories and is gaining increasing use in clinical medicine and pathology. The technique enables multiple simultaneous light scatter and fluorescence measurements to be made at the individual cell level at very rapid rates and results in very large quantities of data being collected. Data, however, is just a series of numbers which have to be converted to information which, in turn, must be shown to have meaning. This is the most important single aspect of flow cytometry but it has received relatively little attention. One of the frequently voiced advantages of the technology is that it produces 'good statistics' because large numbers of cells have been analysed. However, it is not very often that confidence limits are placed on results, hence the reader has little or no feel for the inherent variability in the information produced. This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analysing dynamic processes. All those who use flow cytometry in their research will find this book an invaluable guide to interpreting the data produced by flow cytometers.
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Specificații

ISBN-13: 9780521019705
ISBN-10: 0521019702
Pagini: 300
Ilustrații: 139 b/w illus. 41 tables
Dimensiuni: 155 x 230 x 19 mm
Greutate: 0.44 kg
Ediția:Revised
Editura: Cambridge University Press
Colecția Cambridge University Press
Locul publicării:Cambridge, United Kingdom

Cuprins

1. Introduction; 2. Fundamental concepts; 3. Probability functions; 4. Significance testing and fit criteria; 5. Regression analysis; 6. Flow cytometric sources of variation; 7. Immunofluorescence data; 8. DNA histogram analysis; 9. Cell cycle kinetics; 10. Dynamic cellular events; 11. Multi-variant analysis primer; 12. Epilogue; Appendices; References; Index.

Recenzii

"I recommend this book as an introduction to statistics for the mathematically intimidated and as a gateway into flow cytometry for the mathematically sophisticated.....the book should prove useful." Cancer Bulletin

Descriere

Shows how various categories of flow-cytometry data can be analysed to produce useful information.