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Genetic Library Construction and Screening: Advanced Techniques and Applications: Springer Lab Manuals

Editat de R.C. Bird, Bruce F. Smith
en Limba Engleză Paperback – 6 noi 2012

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Specificații

ISBN-13: 9783642477331
ISBN-10: 364247733X
Pagini: 256
Ilustrații: XIII, 241 p.
Dimensiuni: 155 x 235 x 13 mm
Greutate: 0.36 kg
Ediția:2002
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Springer Lab Manuals

Locul publicării:Berlin, Heidelberg, Germany

Public țintă

Professional/practitioner

Cuprins

PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning — Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.

Caracteristici

A comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic libraries Detailed step-by-step protocols and extensive hands-on advice gleaned from the authors' experience Perfectly suited for experienced researchers and graduate students using these technologies in the lab Includes supplementary material: sn.pub/extras