Genetic Preservation of Plant Cells in Vitro: Springer Lab Manuals
Editat de Brian Grouten Limba Engleză Paperback – iul 2012
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Specificații
ISBN-13: 9783642489686
ISBN-10: 3642489680
Pagini: 184
Ilustrații: XI, 169 p. 22 illus.
Dimensiuni: 189 x 246 x 10 mm
Greutate: 0.34 kg
Ediția:1995
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Springer Lab Manuals
Locul publicării:Berlin, Heidelberg, Germany
ISBN-10: 3642489680
Pagini: 184
Ilustrații: XI, 169 p. 22 illus.
Dimensiuni: 189 x 246 x 10 mm
Greutate: 0.34 kg
Ediția:1995
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Springer Lab Manuals
Locul publicării:Berlin, Heidelberg, Germany
Public țintă
Professional/practitionerCuprins
1 Introduction to the in Vitro Preservation of Plant Cells, Tissues and Organs.- 1.1 Storage Techniques.- 1.2 Principles of Cryogenic Storage.- References.- 2 Minimal Growth Storage.- 2.1 Growth at Reduced Temperature.- 2.2 The Addition of Inhibitory Growth Regulators.- 2.3 The Addition of Osmotic Regulators.- 2.4 Combined Treatment.- References.- 3 Cryopreservation of Protoplast, Suspension and Callus Cultures.- 3.1 General Practical Considerations.- 3.2 Protoplast Cryopreservation by Slow Cooling.- 3.3 Cryopreservation of Cell Suspension Cultures by Slow Cooling.- 3.4 Cryopreservation of Callus Cultures by Slow Cooling.- References.- 4 Cryopreservation of Excised Meristems, Shoot Tips and Somatic Embryos.- 4.1 Meristem/Shoot-Tip Cryopreservation by Slow Cooling.- 4.2 Meristem/Shoot-Tip Cryopreservation by Rapid Cooling.- 4.3 Cryopreservation of Somatic Embryos by Dry Freezing.- 4.4 Cryopreservation of Somatic Embryos by Rapid Cooling.- 4.5 Cryopreservation of Somatic Embryos Following Dessication.- References.- 5 Storage of Free Pollen, Pollen Embryos and the Zygotic Embryos of Seed by Cryopreservation and Freeze Drying.- 5.1 Freezing Drying of Pollen.- 5.2 Pollen Cryopreservation.- 5.3 Cryopreservation of Pollen Embryos.- 5.4 Cryopreservation of Excised Zygotic Embryos.- References.- 6 Conservation of Algae.- 6.1 Serial Subculture.- 6.2 Cryopreservation.- References.- 7 Cryopreservation by Vitrification.- 7.1 Vitrification of Shoot Tips.- 7.2 Encapsulation/Dehydration Method.- 7.3 Vitrification of Cells and Protoplasts.- 7.4 Further Experiments.- References.- 8 Biochemical and Molecular Methods for Assessing Damage, Recovery and Stability in Cryopreserved Plant Germplasm.- A Biochemical Methods.- B Molecular Methods.- References.
Recenzii
"Each chapter is written as a "hands-on" practical guide and presents procedures that can be adapted to individual laboratory conditions and modified to best suit the particular material being preserved." Bioscope