Handbook of Cryo-Preparation Methods for Electron Microscopy: Methods in Visualization
Editat de Annie Cavalier, Daniele Spehner, Bruno M. Humbelen Limba Engleză Hardback – 2 oct 2008
Contributions to this handbook –
Ö Describe cryo-methods aimed at perfect preservation for fine structural analysis
Ö Teach how to arrest physiological processes by cryo-fixation
Ö Reveal the secrets for high-resolution snapshots of life by CEMOVIS
Ö Cover applications of cryo-methods such as electron crystallography, 2D/3D structure analysis, protein localization, and cryo-electron tomography
Ö Demonstrate the use of vitreous water as an intermediate step for localization of biological ions and molecules
Ö Present hybrid methods of freeze-substitution and freeze-drying for immunolabeling and determining molecular geography
Ö Illustrate freeze-fracture, cryo-ultramicrotomy, and resin embedding
Ö Highlight the Tokuyasu method and the new rehydration technique for highly efficient immunolabeling
Ö Include summary tables to compare and obtain appropriate criteria
Includes a wealth of immediate reference material for daily use
With clear, step-by-step recipes and lists of tools, ingredients, and suppliers, this handbookprovides researchers with the knowledge and techniques to adopt the latest cryo-methods to their current research.
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Specificații
ISBN-13: 9780849372278
ISBN-10: 0849372275
Pagini: 706
Ilustrații: 406 b/w images, 2 tables and 300 halftones
Dimensiuni: 178 x 254 x 36 mm
Greutate: 1.38 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Methods in Visualization
Locul publicării:Boca Raton, United States
ISBN-10: 0849372275
Pagini: 706
Ilustrații: 406 b/w images, 2 tables and 300 halftones
Dimensiuni: 178 x 254 x 36 mm
Greutate: 1.38 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Methods in Visualization
Locul publicării:Boca Raton, United States
Public țintă
ProfessionalCuprins
Cryo-Fixation Methods. Macromolecular Assemblies. Cryo-ultramicrotomy. Low-Temperature Embedding. Freeze-Fracture and Metal Shadowing. Immunolabeling, Electron Tomography.
Descriere
Documenting the exponential growth in cryo-electron microscopy, the resulting advances, new hypotheses, and heightened understanding of the ultrastructure of living and inert matter, this book employes an accessible, step-by-step format to present the most sophisticated methods used in research laboratories today. It describes the basic principles of cryo-fixation, cryo-ultramicrotomy, embedding at low temperatures, and freeze-fracture. It explores applications in electron crystallography, 2D/3D structure analysis, cryo-electron tomography, and techniques for protein localization. Each chapter covers a specific method, including detailed protocols, summary tables, and hundreds of illustrations and diagrams.