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Visualization of Receptors In Situ: Applications of Radioligand Binding: Methods in Visualization

Autor Emmanuel Moyse, Slavica M Krantic
en Limba Engleză Hardback – 30 noi 2000
Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures - often not encountered in the original research papers - helping the reader overcome the challenges associated with this powerful technique.
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Specificații

ISBN-13: 9780849300424
ISBN-10: 0849300428
Pagini: 204
Ilustrații: 7 tables, 19 halftones and 23 equations
Dimensiuni: 178 x 254 x 16 mm
Greutate: 0.59 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Methods in Visualization


Public țintă

Professional

Cuprins

LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.

Notă biografică

Emmanuel Moyse, Slavica M Krantic

Descriere

Radioligand binding in situ is the most sensitive method for detecting available receptors; the success and reliability of this approach are determined by a number of technical requirements and constraints. Based on the authors' fifteen years of experience in the field, Visualization of Receptors In Situ: Applications of Radioligand Binding explicitly details these procedures and provides step-by-step methods for visualizing receptors via radioligand binding - information not found in primary research materials. It covers many practical procedures, including radioligand and tissue preparations, in vitro assay, autoradiographic emulsion handling, and image analysis.