PCR/RT- PCR in situ: Light and Electron Microscopy: Methods in Visualization
Autor Gerard Morel, Mireille Raccurten Limba Engleză Hardback – 27 sep 2002
PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.
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Specificații
ISBN-13: 9780849300417
ISBN-10: 084930041X
Pagini: 444
Ilustrații: 237 b/w images, 21 color images and 24 halftones
Dimensiuni: 178 x 254 x 29 mm
Greutate: 1.01 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Methods in Visualization
ISBN-10: 084930041X
Pagini: 444
Ilustrații: 237 b/w images, 21 color images and 24 halftones
Dimensiuni: 178 x 254 x 29 mm
Greutate: 1.01 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Seria Methods in Visualization
Public țintă
ProfessionalCuprins
General Introduction Chapter 1 - General Principles Chapter 2 - Preparation of Samples Chapter 3 - Pretreatments Chapter 4 - Reverse Transcription (RT) Chapter 5 - Polymerase Chain Reation (PCR) Chapter 6 - Hybridization Chapter 7 - Revelation Chapter 8 - Electron Microscopy Chapter 9 - Controls and Problems Chapter 10 - Typical Protocols Chapter 11 - Examples of Observations
Notă biografică
Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.
Descriere
This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.