Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function: Methods in Enzymology, cartea 654
Daniel L. Minor, Henry M. Colecraften Limba Engleză Hardback – 14 iun 2021
Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
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Specificații
ISBN-13: 9780128239247
ISBN-10: 0128239247
Pagini: 446
Dimensiuni: 152 x 229 mm
Greutate: 0.77 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
ISBN-10: 0128239247
Pagini: 446
Dimensiuni: 152 x 229 mm
Greutate: 0.77 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
Public țintă
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.Cuprins
Preface
Daniel L. Minor Jr. and Henry M. Colecraft
Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.
1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern
2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless
3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung
4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura
5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx
Methods to control and characterize ion channel function
6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft
7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny
8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein
9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl
10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU
11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS
Methods to characterize ion channel function and expression in physiology and disease
12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko
13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea
14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge
15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang
16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.
17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy
Nenad Bursac
Daniel L. Minor Jr. and Henry M. Colecraft
Methods to engineer ion channels to probe different aspects of channel structure-function and regulation.
1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern
2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless
3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung
4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura
5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx
Methods to control and characterize ion channel function
6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft
7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny
8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein
9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl
10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU
11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS
Methods to characterize ion channel function and expression in physiology and disease
12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko
13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea
14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge
15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang
16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr.
17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapy
Nenad Bursac