Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis: Oily Press Lipid Library Series
Autor W. W. Christie, X Hanen Limba Engleză Paperback – 18 aug 2016
Lipid analysis deals with the isolation, separation, identification and structural analysis of glycerolipids, including triacylglycerols, phospholipids, sphingolipids, and the various hydrolysis products of these. The chapters follow a logical sequence from the extraction of lipids to the isolation and characterization of particular lipid classes and of molecular species of each, and to the mass spectrometric analysis of lipids and lipidomics.
The new influence of mass spectrometry is due mainly to the development of electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI). Most emphasis in this book is placed on ESI, which is enabling structural characterization of different lipid classes and the identification of novel lipids and their molecular species.
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Specificații
ISBN-13: 9780081014653
ISBN-10: 0081014651
Pagini: 448
Dimensiuni: 156 x 234 x 23 mm
Greutate: 0.62 kg
Ediția:Revised.
Editura: ELSEVIER SCIENCE
Seria Oily Press Lipid Library Series
ISBN-10: 0081014651
Pagini: 448
Dimensiuni: 156 x 234 x 23 mm
Greutate: 0.62 kg
Ediția:Revised.
Editura: ELSEVIER SCIENCE
Seria Oily Press Lipid Library Series
Cuprins
Part 1 introducing lipids and their analysis: Lipids: Their structures and occurrence; Chromatographic analysis of lipids: General principles; Lipid extraction, storage and sample handling. Part 2 chromatographic separation and analysis of individual lipid classes: Analysis of simple lipid classes; Chromatographic analysis of phospholipids and glycosyldiacylglycerols; Chromatographic analysis of sphingolipids. Part 3 analysis of fatty acids: Preparation of derivatives of fatty acids; Gas chromatographic analysis of fatty acid derivatives; Isolation of fatty acids and identification by spectroscopic and related techniques. Part 4 chromatographic analysis of molecular species of lipids: Molecular species of triacylglycerols, diacylglycerols derived from complex lipids, and related lipids; Chromatographic analysis of molecular species of intact phospholipids and glycolipids; Positional distributions of fatty acids in glycerolipids. Part 5 mass spectrometric analysis of lipids in lipidomics: Introduction to mass spectrometric analysis of lipids in lipidomics; Characterization of lipids by electrospray ionization mass spectrometry; Practical identification of individual lipid species in lipid extracts of biological samples; Quantification of lipid molecular species by electrospray ionization mass spectrometry.