Methods in Methane Metabolism, Part B: Methanotrophy: Methods in Enzymology, cartea 495
Editat de Amy Rosenzweig, Stephen W. Ragsdaleen Limba Engleză Hardback – 16 mai 2011
- Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems and field measurements
- The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field
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Specificații
ISBN-13: 9780123869050
ISBN-10: 0123869056
Pagini: 360
Dimensiuni: 152 x 229 x 25 mm
Ediția:New.
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
ISBN-10: 0123869056
Pagini: 360
Dimensiuni: 152 x 229 x 25 mm
Ediția:New.
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
Public țintă
Researchers and the wider scientific communityCuprins
1. Characterization of the Recombinant Pyrophosphate-Dependent 6-Phosphofructokinases from Methylomicrobium alcaliphilum 20Z and Methylococcus capsulatus Bath 2. Genes and Enzymes of Ectoine Biosynthesis in Halotolerant Methanotrophs 3. Facultative And Obligate Methanotrophs: How To Identify And Differentiate Them 4. Approaches for the Characterization and Description of Novel Methanotrophic Bacteria 5. Methylococcus capsulatus (Bath): From Genome to protein function, and vice versa 6. Functional metagenomics of methylotrophs 7. Genetic Systems for Moderately Halo(alkali)philic Bacteria of the genus Methylomicrobium 8. Development of a system for genetic manipulation of the facultative methanotroph Methylocella silvestris BL2 9. Mutagenesis of soluble methane monooxygenase 10. Single cell methods for methane oxidation analysis 11. Methanotroph Outer Membrane Preparation 12. Over-Expression And Purification Of The Particulate Methane Monooxygenase From Methylococcus Capsulatus (Bath) 13. Metal reconstitution of particulate methane monooxygenase and heterologous expression of the pmoB subunit 14. Particulate Methane Monooxygenase From Methylosinus Trichosporium Ob3b 15. Production, Isolation, Purification and Functional Characterisation of Methanobactins 16. A simple assay for screening microorganisms for chalkophore production 17. Isolation of Methanobactin From the Spent Media of Methane Oxidizing Bacteria 18. Measurements of biosphere-atmosphere exchange of CH4 in terrestrial ecosystems