Molecular Characterization of Autophagic Responses Part B: Methods in Enzymology, cartea 588
Lorenzo Galluzzi, Guido Kroemer, Jose Manuel Bravo-San Pedroen Limba Engleză Hardback – 22 feb 2017
Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional.
- Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responses
- Written in an accessible style by renowned experts in the field
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Specificații
ISBN-13: 9780128096741
ISBN-10: 0128096748
Pagini: 608
Dimensiuni: 152 x 229 mm
Greutate: 0.98 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
ISBN-10: 0128096748
Pagini: 608
Dimensiuni: 152 x 229 mm
Greutate: 0.98 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology
Public țintă
Students and entry-level scientists who are for the first time approaching the study of autophagy as well as experienced researchers.Cuprins
1. Renilla Luciferase-LC3 Based Reporter Assay for Measuring Autophagic Flux
2. Measurement of Autolysosomal pH by Dual-Wavelength Ratio Imaging
3. Long-Lived Protein Degradation During Autophagy
4. Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncanonical Amino Acid Tagging
5. Methods to Monitor and Manipulate TFEB Activity During Autophagy
6. Application of CRISPR/Cas9 to Autophagy Research
7. A Molecular Reporter for Monitoring Autophagic Flux in Nervous System In Vivo
8. Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy
9. Assessment of Glycolytic Flux and Mitochondrial Respiration in the Course of Autophagic Responses
10. Methods to Assess Mitochondrial Morphology in Mammalian Cells Mounting Autophagic or Mitophagic Responses
11. Monitoring Mitophagy in Mammalian Cells
12. Cytofluorometric Assessment of Mitophagic Flux in Mammalian Cells and Tissues
13. Automated Analysis of Fluorescence Colocalization: Application to Mitophagy
14. Assays to Monitor Lysophagy
15. Kinetics of Protein Aggregates Disposal by Aggrephagy
16. Methods to Study Chaperone-Mediated Autophagy
17. Quantitative Assay of Macroautophagy Using Pho8△60 Assay and GFP-Cleavage Assay in Yeast
18. Monitoring the Formation of Autophagosomal Precursor Structures in Yeast Saccharomyces cerevisiae
19. Methods to Assess Autophagy and Chronological Aging in Yeast
20. Methods to Measure Lipophagy in Yeast
21. Assays to Monitor Pexophagy in Yeast
22. Monitoring Autophagic Responses in Caenorhabditis elegans
23. Characterization of Autophagic Responses in Drosophila melanogaster
24. Methods to Study Autophagy in Zebrafish
25. Biochemical Methods to Monitor Autophagic Responses in Plants
26. Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants
2. Measurement of Autolysosomal pH by Dual-Wavelength Ratio Imaging
3. Long-Lived Protein Degradation During Autophagy
4. Proteomic Profiling of De Novo Protein Synthesis in Starvation-Induced Autophagy Using Bioorthogonal Noncanonical Amino Acid Tagging
5. Methods to Monitor and Manipulate TFEB Activity During Autophagy
6. Application of CRISPR/Cas9 to Autophagy Research
7. A Molecular Reporter for Monitoring Autophagic Flux in Nervous System In Vivo
8. Magnetic Resonance Spectroscopy to Study Glycolytic Metabolism During Autophagy
9. Assessment of Glycolytic Flux and Mitochondrial Respiration in the Course of Autophagic Responses
10. Methods to Assess Mitochondrial Morphology in Mammalian Cells Mounting Autophagic or Mitophagic Responses
11. Monitoring Mitophagy in Mammalian Cells
12. Cytofluorometric Assessment of Mitophagic Flux in Mammalian Cells and Tissues
13. Automated Analysis of Fluorescence Colocalization: Application to Mitophagy
14. Assays to Monitor Lysophagy
15. Kinetics of Protein Aggregates Disposal by Aggrephagy
16. Methods to Study Chaperone-Mediated Autophagy
17. Quantitative Assay of Macroautophagy Using Pho8△60 Assay and GFP-Cleavage Assay in Yeast
18. Monitoring the Formation of Autophagosomal Precursor Structures in Yeast Saccharomyces cerevisiae
19. Methods to Assess Autophagy and Chronological Aging in Yeast
20. Methods to Measure Lipophagy in Yeast
21. Assays to Monitor Pexophagy in Yeast
22. Monitoring Autophagic Responses in Caenorhabditis elegans
23. Characterization of Autophagic Responses in Drosophila melanogaster
24. Methods to Study Autophagy in Zebrafish
25. Biochemical Methods to Monitor Autophagic Responses in Plants
26. Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants
Recenzii
Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience