Reviews and Protocols in DT40 Research: Subcellular Biochemistry: Subcellular Biochemistry, cartea 40
Editat de Jean-Marie Buerstedde, Shunichi Takedaen Limba Engleză Hardback – 25 oct 2006
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Specificații
ISBN-13: 9781402048951
ISBN-10: 1402048955
Pagini: 492
Ilustrații: XII, 477 p.
Dimensiuni: 210 x 297 x 36 mm
Greutate: 0.82 kg
Ediția:2006
Editura: SPRINGER NETHERLANDS
Colecția Springer
Seria Subcellular Biochemistry
Locul publicării:Dordrecht, Netherlands
ISBN-10: 1402048955
Pagini: 492
Ilustrații: XII, 477 p.
Dimensiuni: 210 x 297 x 36 mm
Greutate: 0.82 kg
Ediția:2006
Editura: SPRINGER NETHERLANDS
Colecția Springer
Seria Subcellular Biochemistry
Locul publicării:Dordrecht, Netherlands
Public țintă
ResearchCuprins
DT40 gene disruptions: a how-to for the design and the construction of targeting vectors.- Immunoglobulin gene conversion or hypermutation: that's the question.- Genome resources for the DT40 community.- Chromosome engineering in DT40 cells and mammalian centromere function.- Function of RECQ family helicase in genome stability.- Genetic analysis of apoptotic execution.- The DT40 system as a tool for analyzing kinetochore assembly.- Analysing the DNA damage and replication checkpoints in DT40 cells.- Using DT40 to study clathrin function.- Genetic analysis of B cell signaling.- DT40 mutants: a model to study transcriptional regulation of B cell development and function.- Transcription and RNA processing factors play complex roles in DT40 cells.- Participation of histones, histone modifying enzymes and histone chaperonesin vertebrate cell functions.- Analysis of gene expression, copy number and palindrome formation with a DT40 enriched CDNA microarray.- Calcium signaling, ion channels and more.- Analysis of DNA replication damage bypass and its role in immunoglobulin repertoire development.- The fanconi anemia pathway promotes homologous recombination repair in DT40 cell line.- Phenotypic analysis of cellular responses to DNA damage.- ATM, a paradigm for a stress-responsive signal transducer in higher vertebrate cells.- Stable non-targeted transfection of DT40.- Basic cell culture conditions.- Excision of floxed-DNA sequences by transient induction of MER-CRE-MER.- Immunoglobulin gene conversion and hypermutation assay by facs.- Target screening by PCR.- Mitotic index determination by flow cytometry.- Centrifugal elutriation as a means of cell cycle phase separation and synchronisation.- Preparation of genomic DNA for microarray-based comparative genome hybridization.- Analysis of cellular Mg2+ in DT40 cells.- Transient transfection of DT40.- Retroviral transduction of DT40.- Colony survival assay.- Subcloning DT40 by limiting dilution.- Subnuclear immunofluorescence.- Sister chromatid exchange assay.- 2D cell cycle analysis.- Purification of tap-tagged proteins by two-step pull down from DT40 cells.- Synchronization of cells.- Targeted transfection of DT40 cells.- Luciferase reporter assay.- Indirect immunofluorescence microscopy.- Quantification of receptor-mediated endocytosis.- Measurement of DNA synthesis and strand breaks using alkaline sucrose density gradient centrifugation.- Isolation of nuclear and cytoplasmic proteins from DT40 cell lines.
Caracteristici
State-of-the-art reviews Protocols to help you succeed Frontiers in DT40 science