Cellular Senescence: Methods and Protocols: Methods in Molecular Biology, cartea 1896
Editat de Marco Demariaen Limba Engleză Hardback – 24 noi 2018
Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Specificații
ISBN-13: 9781493989300
ISBN-10: 1493989308
Pagini: 220
Ilustrații: XII, 253 p. 46 illus., 39 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.69 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 1493989308
Pagini: 220
Ilustrații: XII, 253 p. 46 illus., 39 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.69 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Cuprins
Detecting Cellular Senescence in Reprogramming.- DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA).- Reactive Oxygen Species Detection in Senescent Cells.- Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo.- Relative Human Telomere Length Quantification by Real-time PCR.- Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP).- Measuring the Inflammasome in Oncogene-induced Senescence.- Alarmin Detection in Senescent Cells.- IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence.- Genotoxic Stress-induced Senescence.- A Multi-parametric Assay to Evaluate Senescent Cells.- A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids.- Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry.- Quantification of Autophagy during Senescence.- Quantification of Aneuploidy in Mammalian Systems.- Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis.- Mouse Models of Accelerated Cellular Senescence.- Methods to Quantify the NF-kB Pathway during Senescence.
Textul de pe ultima copertă
This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts