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Nucleic Acid and Peptide Aptamers: Methods and Protocols: Methods in Molecular Biology, cartea 535

Editat de Günter Mayer
en Limba Engleză Hardback – 26 mar 2009
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
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Specificații

ISBN-13: 9781934115893
ISBN-10: 1934115894
Pagini: 424
Ilustrații: XI, 408 p.
Dimensiuni: 193 x 260 x 28 mm
Greutate: 1 kg
Ediția:2009
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

I Nucleic Acid Aptamers.- Nucleic Acid Pool Preparation and Characterization.- In Vitro Selection of ssDNA Aptamers Using Biotinylated Target Proteins.- Isolating Aptamers Using Capillary Electrophoresis–SELEX (CE–SELEX).- In Vitro Selection of Allosteric Ribozymes.- Cell-Specific Aptamers for Targeted Therapies.- Aptamers Targeting RNA Molecules.- Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction.- Structural Probing Techniques on Natural Aptamers.- Determining Structures of RNA Aptamers and Riboswitches by X-Ray Crystallography.- Locked Nucleic Acid Aptamers.- Screening of Novel Inhibitors of HIV-1 Reverse Transcriptase with a Reporter Ribozyme Assay.- Aptamers as Artificial Gene Regulation Elements.- Aptamers and Biosensors.- Nanoparticles/Dip Stick.- In Vivo Imaging of Oligonucleotidic Aptamers.- II Peptide Aptamers.- Selection of Peptides Interfering with Protein–Protein Interaction.- In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.- Ligand-Regulated Peptide Aptamers.- Isolation of Peptide Aptamers to Target Protein Function.- Microbodies™.- Peptide Aptamers for Small Molecule Drug Discovery.- Synthesis and Application of Peptides as Drug Carriers.

Recenzii

From the reviews:
"The book ‘Nucleic Acids and Peptide Aptamers’ is a collection of detailed protocols describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. … The list of references is a useful and timely source of further reading on the subject. … can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)

Textul de pe ultima copertă

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Caracteristici

Provides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field Covers a wide range of in vitro selection protocols targeting different molecules Comprehensive guide to identifying your specific target binding aptamer, whether of nucleic acid or peptidic nature Detailed presentation of applications of aptamers The first comprehensive summary that covers peptide and nucleic acid aptamers in one volume Includes supplementary material: sn.pub/extras