Endocannabinoid Signaling: Methods and Protocols: Methods in Molecular Biology, cartea 1412
Editat de Mauro Maccarroneen Limba Engleză Hardback – iun 2016
Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists,molecular biologists, cell biologists, pharmacologists, and (electro)physiologists navigate the mare magnum of endocannabinoid research.
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Specificații
ISBN-13: 9781493935376
ISBN-10: 1493935372
Pagini: 260
Ilustrații: XI, 285 p. 75 illus., 26 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.94 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
ISBN-10: 1493935372
Pagini: 260
Ilustrații: XI, 285 p. 75 illus., 26 illus. in color.
Dimensiuni: 178 x 254 x 22 mm
Greutate: 0.94 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:New York, NY, United States
Public țintă
Professional/practitionerCuprins
Need for Methods to InvestigateEndocannabinoid Signaling.- Extraction and Simultaneous Quantificationof Endocannabinoids and Endocannabinoid-Like Lipidsin Biological Tissues.- Determination of 2-Arachidonoylglycerol by µSPE-LC-MS/MS.- Analysis of Omega-3 Fatty AcidDerived N-acylethanolamines in BiologicalMatrices.- Assay of CB1 Receptor Binding.- The Displacement Binding Assay Using HumanCannabinoid CB2 Receptor-Transfected Cells.- Assay of TRPV1 Receptor Signaling.- A Functional Assay for GPR55: Envision Protocols.- The Cyclic AMP Assay Using Human CannabinoidCB2 Receptor-Transfected Cells.- Assay of GTPγS Binding in Autoradiography.- Protocol to Study β-Arrestin Recruitment by CB1 and CB2Cannabinoid Receptors.- Assay of NAT Activity.- Assay of NAPE-PLD Activity.- Assay of FAAH Activity.- Assay of NAAA Activity.- Assay of DAGLα/β Activity.- Assay of Monoacylglycerol Lipase Activity.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD6.- A Sensitive and Versatile Fluorescent Activity Assay for ABHD12.- Assay of Endocannabinoid Uptake.- Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.- Oxygenation of Anandamide by Lipoxygenases.- Assay of Endocannabinoid Oxidation by Cytochrome P450.- Assessing Gene Expression of the Endocannabinoid System.- Western Blotting of the Endocannabinoid System.- Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Traffickingin Cultured Cells.- Measuring ECS Interaction with Biomembranes.- Visualization of Endocannabinoids in the Cell.
Textul de pe ultima copertă
Thisvolume encompasses all major methodologies to interrogate endocannabinoidsystems (ECS) and endocannabinoids (eCBs) signaling. With increasing interesttowards the manifold activities of eCBs, this book discusses the chemical,biochemical, and molecular biological assays, and activity of distinct elementsof the ECS. These include membrane, nuclear receptors, biosynthetic andhydrolytic enzymes, and membrane transporters and oxidative enzymes. Written inthe highly successful Methods in Molecular Biology series format,chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists,molecular biologists, cell biologists, pharmacologists, and (electro)physiologists navigate the mare magnum of endocannabinoid research.
Timely and cutting edge, Endocannabinoid Signaling: Methods and Protocolsis a valuable resource and will help chemists, drug designers, biochemists,molecular biologists, cell biologists, pharmacologists, and (electro)physiologists navigate the mare magnum of endocannabinoid research.
Caracteristici
Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implelementation advice from the experts