Cantitate/Preț
Produs
Total produse
Vezi coșul
Estrogen Receptors: Methods and Protocols de Kathleen M. Eyster

Estrogen Receptors: Methods and Protocols: Methods in Molecular Biology, cartea 1366

Editat de Kathleen M. Eyster
en Limba Engleză Hardback – 20 noi 2015
This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.
The chapter 'Bioinformatics Analysisof Estrogen-Responsive Genes' is open access under a CC BY 4.0 license. 

Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (2) 68535 lei  38-44 zile
  Springer – 23 aug 2016 68535 lei  38-44 zile
  Springer Us – 5 feb 2023 93695 lei  6-8 săpt.
Hardback (2) 96497 lei  3-5 săpt.
  Springer – 20 noi 2015 96497 lei  3-5 săpt.
  Springer Us – 5 feb 2022 136731 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 60% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 34795 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59856 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49679 lei
  • 20% Clinical Bioinformatics
    Preț: 88295 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 71488 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71665 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72633 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 73206 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 65004 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 100455 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 71971 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 89567 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 65117 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 64089 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 138603 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 72759 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 95259 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64715 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 72348 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 71846 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39258 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 73117 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 94315 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86021 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 63944 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 103411 lei
  • 23% Computational Protein Design
    Preț: 88385 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79216 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42362 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42591 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59220 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103884 lei
  • 5% Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications
    Preț: 52415 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 207922 lei
  • 5% Epitope Mapping Protocols
    Preț: 127289 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78993 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133910 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 136207 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75266 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37489 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 136731 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 110674 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 137969 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 110211 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 94669 lei

Preț: 96497 lei

Preț vechi: 117680 lei
-18% Nou

Puncte Express: 1447
Preț estimativ în valută:
18468€ • 19183$ • 15340£

Carte disponibilă

Livrare economică 11-25 ianuarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493931262
ISBN-10: 1493931261
Pagini: 576
Ilustrații: XVI, 576 p. 128 illus., 87 illus. in color.
Dimensiuni: 178 x 254 x 30 mm
Greutate: 1.58 kg
Ediția:1st ed. 2016
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

The Estrogen Receptors: An Overview from Different Perspectives.- Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER).- The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression.- Bioinformatics Analysis of Estrogen-Responsive Genes.- Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen.- Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors.- Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells.- Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer.- RNA-Seq Experiment and Data Analysis.- DNA Microarray Analysis of Estrogen-Responsive Genes.- Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry.- Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen.- Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy.- Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy.- In Situ Hybridization of Estrogen Receptors α and β and GPER in the Human Testis.- Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a.- Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction.-  Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy.- Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin.- Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor α and Specificity Protein 1 with Composite DNA Elements.- Tips and Tricks for Successful Application of Statistical Methods to Biological Data.- The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites.- Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model.- Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model.- Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo Culture.- Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay.- Silencing Estrogen Receptor-α with siRNA inthe Intact Rodent Brain.- Silencing Estrogen Receptor-β with siRNA in Cultured Cells.- Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists.- Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells.- Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR.-Detection and Functional Analysis of Estrogen Receptor α Phosphorylated at Serine 216 in Mouse Neutrophils.- Estrogen Receptor-α Knockout Mice.- Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol.- Phytoestrogens Activate the Estrogen Receptor in HepG2 Cells.- Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation.- GPER Mediates Non-Genomic Effects of Estrogen.- GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism.- Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation.- Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods.- Assessing Direct Vascular Actions of Estrogens.- Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERα.- Regulation of Activation Induced Deaminase (AID) by Estrogen.

Notă biografică

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

Textul de pe ultima copertă

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.
 

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras