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Constitutive Activity in Receptors and Other Proteins, Part B de Melvin I. Simon

Constitutive Activity in Receptors and Other Proteins, Part B: Methods in Enzymology, cartea 485

Melvin I. Simon
en Limba Engleză Hardback – 24 noi 2010
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume.


  • Expert authors who are leaders in the field
  • Extensively referenced and useful figures and tables
  • Provides hints and tricks to facilitate reproduction of methods
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Specificații

ISBN-13: 9780123812964
ISBN-10: 0123812968
Pagini: 708
Dimensiuni: 152 x 229 x 33 mm
Greutate: 1.11 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Enzymology

Public țintă

Researchers and students in cell, molecular and developmental biology

Cuprins

    1. Identification and characterization of Steroidogenic Factor-1 inverse agonists. Mabrouka Doghman, Franck Madoux, Peter Hodder, and Enzo Lalli
    2. Assessment of inverse agonism for the angiotensin II type 1 receptor Hiroshi Akazawa, Noritaka Yasuda, Shin-ichiro Miura, Issei Komuro
    3. Measurement of inverse agonism in BARs Carlos Alberto Taira
    4. Inverse agonism of antidepressants: in vitro and in vivo studies Joel Bockaert
    5. Differential inverse agonism at the human muscarinic M3 receptor Paola Casarosa
    6. Ghrelin Receptor: High Constitutive Activity and Methods for Developing Inverse Agonists. Constance Chollet
    7. Constitutive activity and inverse agonism at the α1a and α1b adrenergic receptor subtypes. Susanna. Cotecchia
    8. Measurement of inverse agonism of the cannabinoid receptor Tung Fong
    9. Constitutively Active Thyrotropin (TSH) and Thyrotropin-Releasing Hormone (TRH) Receptors and Their Inverse Agonists Marvin C. Gershengorn
    10. Inverse retinoid agonists and neutral antagonists Hinrich Gronemeyer
    11. γ-Aminobutyric Acid Type A (GABAA) Receptor Subtype Inverse Agonists as Therapeutic Agents in Cognition Gabriella Guerrini and Ciciani Giovanna
    12. Assays for inverse agonists in the visual system. Masahiro Kono
    13. Receptor-driven identification of novel human A3 adenosine receptor antagonists as potential therapeutic agents Silvia Paoletta, Stephanie Federico, Giampiero Spalluto and Stefano Moro
    14. Inverse agonists of steroidogenic factor SF-1 Fabrice Piu and Andria L. Del Tredici
    15. Methods to Measure G Protein Coupled Receptor Activity for the Identification of Inverse Agonists Gabriel Barreda-Gómez, M.Teresa Giralt and Rafael Rodríguez-Puertas
      Section II-- Novel Strategies and Techniques for Constitutive Activity and Inverse Agonism
    16. Use of Pharmacoperones to Reveal GPCR Structural Components Associated with Receptor Activation and Trafficking Jo Ann Janovick and P. Michael Conn
    17. Application of large-scale transiently transfected cells to functional assays of ion channels and other proteins. Jun Chen, Sujatha Gopalakrishnan, Marc R Lake, and Bruce R Bianchi
    18. Quantification of RNA Editing of the Serotonin 2C Receptor (5-HT2CR) Ex Vivo Maria Fe Lanfranco, Noelle C. Anastasio, Patricia K. Seitz, and Kathryn A. Cunningham
    19. Strategies for isolating constitutively-active and dominant-negative pheromone receptor mutants in yeast. Mercedes Dosil and James B. Konopka
    20. Development of a GPR23 Cell-based β-Lactamase Reporter Assay Paul H. Lee and Bonnie J. Hanson
    21. Computational Modeling of Constitutively Active Mutants of GPCRs: C5a Receptor. Gregory V. Nikiforovich and Thomas J. Baranski
    22. TSH receptor monoclonal antibodies with agonist, antagonist and inverse agonist activities Jane Sanders, Ricardo Núñez Miguel, Jadwiga Furmaniak, Bernard Rees Smith
    23. Current standards, variations and pitfalls for the determination of constitutive TSHR activity in vitro Sandra Mueller, Holger Jaeschke, Ralf Paschke
    24. Towards the Rational Design of Constitutively Active KCa3.1 Mutant Channels Line Garneau, Hélène Klein, Lucie Parent, Rémy Sauvé
    25. Fusion proteins as model systems for the analysis of constitutive GPCR activity Erich H. Schneider, Roland Seifert
    26. Screening for Novel Constitutively Active CXCR2 Mutants and Their Cellular Effects Giljun Park, Tom Masi, Chang K. Choi, Heejung Kim, Jeffrey M. Becker, and Tim E. Sparer
    27. A method for parallel solid-phase synthesis of iodinated analogues of the cannabinoid receptor type I (CB1) inverse agonist rimonabant Alan C. Spivey and Chih-Chung Tseng
    28. Coexpression systems as models for the analysis of constitutive GPCR activity Roland Seifert and Erich H. Schneider
    29. Modelling and simulation of inverse agonism dynamics Lloyd J. Bridge
    30. Design and Use of Constitutively Active STAT5 Constructs Michael A. Farrar
    31. In vitro and in vivo assays of protein kinase CK2 activity
    Renaud Prudent, Céline F. Sautel, Virginie Moucadel, Béatrice Laudet, Odilhe Filhol, and Claude Cochet