Cantitate/Preț
Produs
Total produse
Vezi coșul
Decoding the Antibody Repertoire: High Throughput Sequencing of Multiple Transcripts from Single B Cells de Brandon DeKosky

Decoding the Antibody Repertoire: High Throughput Sequencing of Multiple Transcripts from Single B Cells: Springer Theses

Autor Brandon DeKosky
en Limba Engleză Hardback – 8 iun 2017
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.
Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells.
The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 68563 lei  43-57 zile
  Springer International Publishing – 4 aug 2018 68563 lei  43-57 zile
Hardback (1) 69220 lei  43-57 zile
  Springer International Publishing – 8 iun 2017 69220 lei  43-57 zile

Din seria Springer Theses

  • 5% sEMG-based Control Strategy for a Hand Exoskeleton System
    Preț: 112094 lei
  • Surface Chemistry and Macroscopic Assembly of Graphene for Application in Energy Storage
    Preț: 37878 lei
  • 15% Search for Scalar Top Quarks and Higgsino-Like Neutralinos: SUSY Hunting With a “Soft” Lepton at the LHC
    Preț: 62841 lei
  • 18% Stable Supported Gold Nanoparticle Catalyst for Environmentally Responsible Propylene Epoxidation
    Preț: 118538 lei
  • Measurement of Neutrino Interactions and Three Flavor Neutrino Oscillations in the T2K Experiment
    Preț: 38792 lei
  • 18% Self-Powered Smart Fabrics for Wearable Technologies
    Preț: 96923 lei
  • 18% Fabrication and Properties of High-Performance 122-Type Iron-Based Superconducting Wires and Tapes
    Preț: 91405 lei
  • Spin Glasses: Criticality and Energy Landscapes
    Preț: 54451 lei
  • 15% Development of Infrared Techniques for Practical Defect Identification in Bonded Joints
    Preț: 62474 lei
  • 15% X-ray Studies of the Central Engine in Active Galactic Nuclei with Suzaku
    Preț: 62428 lei
  • 15% Metamaterials for Manipulation of Thermal Radiation and Photoluminescence in Near and Far Fields
    Preț: 62096 lei
  • 20% Studies on "Perfect" Hyperbranched Chains Free in Solution and Confined in a Cylindrical Pore
    Preț: 55880 lei
  • 18% Detection Efficiency and Bandwidth Optimized Electro-Optic Sampling of Mid-Infrared Waves
    Preț: 91635 lei
  • 18% Analysis, Optimization and Control of Grid-Interfaced Matrix-Based Isolated AC-DC Converters
    Preț: 108420 lei
  • 15% Probing Unconventional Transport Regimes in Delafossite Metals
    Preț: 62174 lei
  • 15% Fluid Flow and Heat Transfer in Porous Media Manufactured by a Space Holder Method
    Preț: 62174 lei
  • Fiber Solar Cells: Materials, Processing and Devices
    Preț: 27668 lei
  • 15% Magnetic Cloud Boundary Layers and Magnetic Reconnection
    Preț: 61822 lei
  • 18% Toroidal Order in Magnetic Metamaterials
    Preț: 86560 lei
  • 15% Probing Cosmic Dark Matter and Dark Energy with Weak Gravitational Lensing Statistics
    Preț: 62252 lei
  • Investigations on Microstructure and Mechanical Properties of the Cu/Pb-free Solder Joint Interfaces
    Preț: 37862 lei
  • 20% Thermospheric Density and Wind Determination from Satellite Dynamics
    Preț: 56388 lei
  • State Estimation and Coordinated Control for Distributed Electric Vehicles
    Preț: 38216 lei
  • 15% Charge and Heat Transport Phenomena in Electronic and Spin Structures in B20-type Compounds
    Preț: 61966 lei
  • 15% Search for Charginos Nearly Mass-Degenerate with the Lightest Neutralino: Based on a Disappearing-Track Signature in pp Collisions at √s = 8 TeV
    Preț: 62348 lei
  • 18% Electroweak Gauginos with Highly Boosted Hadronically Decaying Bosons at the LHC
    Preț: 108035 lei
  • 20% Theory of Bilayer Graphene Spectroscopy
    Preț: 55134 lei
  • 18% Structure and Electronic Properties of Ultrathin In Films on Si(111)
    Preț: 107193 lei
  • 18% Modeling and Control Strategies for a Fuel Cell System
    Preț: 107805 lei
  • 18% Fracture Analysis of Layered Beams With an Elastically Coupled Behavior and Hygrothermal Stresses: Application to Metal-to-Composite Adhesive Joints
    Preț: 119072 lei
  • 18% Phononic and Electronic Excitations in Complex Oxides Studied with Advanced Infrared and Raman Spectroscopy Techniques
    Preț: 91787 lei
  • 18% Atomic and Electronic Properties of 2D Moiré Interfaces
    Preț: 91709 lei
  • 15% Towards THz Chipless High-Q Cooperative Radar Targets for Identification, Sensing, and Ranging
    Preț: 62174 lei
  • 18% Storage Ring-Based Inverse Compton X-ray Sources: Cavity Design, Beamline Development and X-ray Applications
    Preț: 119380 lei
  • 15% Study of Second Generation High Temperature Superconductors: Electromagnetic Characteristics and AC Loss Analysis
    Preț: 62174 lei
  • 18% Microparticle Dynamics in Electrostatic and Flow Fields
    Preț: 118230 lei
  • 15% Electroweak Baryogenesis and Its Phenomenology
    Preț: 61857 lei
  • 18% Realizing an Andreev Spin Qubit: Exploring Sub-gap Structure in Josephson Nanowires Using Circuit QED
    Preț: 97214 lei
  • 15% Super El Niño
    Preț: 61777 lei
  • 15% Spin and Charge Ordering in the Quantum Hall Regime
    Preț: 62252 lei
  • Dynamic Wetting by Nanofluids
    Preț: 37618 lei
  • 18% Structures and Dynamics of Interfacial Water
    Preț: 97076 lei
  • Design, Synthesis, Multifunctionalization and Biomedical Applications of Multifunctional Mesoporous Silica-Based Drug Delivery Nanosystems
    Preț: 37428 lei
  • Investigations on Mesoscale Structure in Gas–Solid Fluidization and Heterogeneous Drag Model
    Preț: 37428 lei
  • 18% Multistep Continuous Flow Synthesis of Fine Chemicals with Heterogeneous Catalysts
    Preț: 107805 lei
  • 18% The Read-Out Controller ASIC for the ATLAS Experiment at LHC
    Preț: 107883 lei
  • Nanostructuring for Nitride Light-Emitting Diodes and Optical Cavities
    Preț: 37597 lei
  • 15% Artificially Controllable Nanodevices Constructed by DNA Origami Technology: Photofunctionalization and Single-Molecule Analysis
    Preț: 61889 lei
  • 20% High-Tc SQUIDs for Biomedical Applications: Immunoassays, Magnetoencephalography, and Ultra-Low Field Magnetic Resonance Imaging
    Preț: 55419 lei
  • 20% Nonlinear Optics in the Filamentation Regime
    Preț: 55556 lei

Preț: 69220 lei

Preț vechi: 72864 lei
-5% Nou

Puncte Express: 1038
Preț estimativ în valută:
13249€ • 13808$ • 11029£

Carte tipărită la comandă

Livrare economică 06-20 ianuarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9783319585178
ISBN-10: 3319585177
Pagini: 87
Ilustrații: XXVIII, 87 p. 34 illus.
Dimensiuni: 155 x 235 mm
Greutate: 0.34 kg
Ediția:1st ed. 2017
Editura: Springer International Publishing
Colecția Springer
Seria Springer Theses

Locul publicării:Cham, Switzerland

Cuprins

Background.- High-throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire.- In-Depth Determination and Analysis of the Human Paired Heavy and Light Chain Antibody Repertoire.- Paired VH:VL Analysis of Naïve B Cell Repertoires and Comparison to Antigen-Experienced B Cell Repertoires in Healthy Human Donors.- Conclusions and Future Perspectives.- Appendices.

Notă biografică

Brandon DeKosky is an assistant professor at the Departments of Pharmaceutical Chemistry, Chemical & Petroleum Engineering, and the Kansas Vaccine Institute at the University of Kansas.  Dr. DeKosky attained his Ph.D. in the lab of George Georgiou at the University of Texas at Austin where he developed new technologies for sequencing complete antibody variable regions from single B cells.  Dr. DeKosky performed postdoctoral studies in the lab of John Mascola at the NIAID Vaccine Research Center, applying high-throughput technologies to accelerate public health vaccine development.  His lab at KU seeks to leverage high-throughput platforms to understand the features of adaptive immune protection and develop novel strategies to combat human diseases.

Textul de pe ultima copertă

This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.

Caracteristici

Nominated as an outstanding Ph.D thesis by the University of Texas at Austin Outlines the development of the very first technology for high-throughput analysis of paired heavy and light chain antibody sequences from Enables comprehensive investigation of human B-cell repertoires for rapid antibody discovery Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras