Non-viral Gene Therapy: Gene Design and Delivery

1 Molecules for Gene Delivery 1-1 Leaf Huang Recent Progress in Nonviral Gene Delivery 1-2 Kenichi Yoshikawa Physical Chemistry of DNA-Carrier Complex 1-3 Kazuo Maruyama Liposome-Mediated Delivery System 1-4 Tomoko Hashimoto Polymeric Gene Carriers 1-5 Kazunori Kataoka Development of Cationic Block Copolymer-Based Gene Delivery System 1-6 Toshinori Sato Chitosan-Mediated Gene Delivery 1-7 Hidetoshi Arima Dendrimers as DNA Carriers1-8 Takuro Niidome Use of Synthetic Peptide for Non-viral Gene Delivery 1-9 Kazuo Sakurai Oligonucleotide Carrier Based on b-1,3-Glucans1-10 Mahito Nakanishi Biological and Chemical Hybrid Vectors 2 Controlled Gene Delivery 2-1 Hideyoshi Harashima Pharmacokinetics of Gene Delivery in Cells2-2 Takeshi Nagasaki Active DNA Release from Complex 2-3 Masayuki Yokoyama Controlled Release of DNA using Thermo-Responsive Polymers 2-4 Takeshi Nagasaki Active Transport of Exogenous Gene into Nucleus2-5 Masayuki Fujii Controlled Intracellular Localization of Oligonucleotides by Chemical Conjugation 2-6 Osam Mazda Functional Nucleotide Sequences Capable of Promoting Nonviral Genetic Transfer2-7 Yoshiyuki Koyama Shielding of cationic charge of the DNA complex to avoid non-specific interactions for in vivo gene delivery 2-8 Shigeru Kawakami In Vivo Gene Transfer by Ligand Modified Gene Carriers 2-9 Ernst Wagner Optimizing Polyplexes into Systemic Viruses for Tumor-Targeted Gene Therapy 2-10 Mariko Harada-Shiba Gene Transfer and Target Diseases 2-11 Hironobu Yanagie Clinical Trials Using Non-Viral Gene Delivery Systems 3 Current Protocoles of Gene Delivery 3-1 Mitsuru Hashida Evaluation of Size and Zeta-Potential of DNA/Carrier Complex 3-2 Tomoko Ito Observation of DNA / Carrier Complex under Fluorescence Microscopy 3-3 Taku Satoh Synthesis of Chemically Modified Chitosan and its Gene Transfection Efficiency 3-4 Hiroyuki Kamiya Newly Designed DNA Fragments for Gene Correcton 3-5 Tatsuya Okuda Evaluation of Gene Expression in Vivo after Intraveous and Intraportal Administration of Lipoplexes3-6 Takuro Niidome Evaluation of DNA Administrated with Gene Carrier in Organs by Southern Blot Hybridization 3-7 Fuminori Sakurai Evaluation of Immune Response after Administration of Plasmid DNA-Nonviral Vector Complex 3-8 Kunt R. Madden Gateway RNAi 4 Design of Genes Based on Current RNA Technology 4-1 Kazunari Taira Design of Intracellulary Active Ribozymes and siRNA 4-2 Takanori Yokota siRNA-based Inhibition Specific for Mutant Allele in Autosomal Dominant Diseases: Sequence-Dependent and -Independent Discriminations of Mutant and Wild-type Alleles by siRNA 4-3 Silvere Petit In Vivo RNA Interference: another tool in the box? 4-4 Hiroaki Kawasaki Suppression of Gene Expression via Chromatin Remodeling and the siRNA-Induced Silencing of Transcription 4-5 Andrew Miller Intracellular delivery of nucleic acids: differences between transfection and siFection reflect differences between DNA and RNA, and between oligodeoxynucleotides and oligonucleotides 4-6 Junichi Yano In Vivo Antitumour Activity of a New Cationic Liposome siRNA Complex 4-7 John Rossi Enhancing RNAi with Synthetic RNA Duplexes

Covers chemical aspects such as design and synthesis of gene carriers, as well as knowledge from in vivo experiments and clinical aspects of non-viral gene therapy
Complementary information of this book, such as movies, experimental data, etc. will be introduced and updated in the web page of The Japanese Society of Gene Design and Delivery