Plant Cell Wall Analysis: Molecular Methods of Plant Analysis, cartea 17
Editat de Hans F. Linskens, John F. Jacksonen Limba Engleză Paperback – oct 2011
Din seria Molecular Methods of Plant Analysis
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Specificații
ISBN-13: 9783642646447
ISBN-10: 3642646441
Pagini: 228
Ilustrații: XVII, 205 p.
Dimensiuni: 155 x 235 x 12 mm
Greutate: 0.33 kg
Ediția:Softcover reprint of the original 1st ed. 1996
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Molecular Methods of Plant Analysis
Locul publicării:Berlin, Heidelberg, Germany
ISBN-10: 3642646441
Pagini: 228
Ilustrații: XVII, 205 p.
Dimensiuni: 155 x 235 x 12 mm
Greutate: 0.33 kg
Ediția:Softcover reprint of the original 1st ed. 1996
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Molecular Methods of Plant Analysis
Locul publicării:Berlin, Heidelberg, Germany
Public țintă
ResearchCuprins
Fractionation of Cell Wall Components.- 1 Introduction.- 2 Polysaccharides.- 3 Proteins and Glycoproteins.- 4 Lignins.- References.- Isolation and Analysis of Cell Wall Polymers from Olive Pulp.- 1 Introduction.- 2 Isolation of Cell Walls from Olive Pulp.- 3 Sequential Extraction of Cell Wall Polymers.- 4 Fractionation of the Extracted Cell Wall Polysaccharides.- 5 Chemical and Spectroscopic Analysis.- 6 Concluding Remarks.- References.- Determination of Cell Wall Autolysis.- 1 Introduction.- 2 Factors Altering Cell Wall Isolation and Autolysis.- 3 Isolation of Active Cell Walls.- 4 Isolation of Inactive Cell Walls (Controls).- 5 Autolysis Incubations.- 6 Analysis of Autolysis Products.- 7 Conclusions.- References.- Cell Wall Porosity and Its Determination.- 1 Introduction.- 2 Microscopic Visualization of Wall Pores.- 3 Bulk Exclusion Techniques.- 4 Tracer Techniques: Uptake of Molecules or Particles.- 5 Conclusions.- References.- Analysis of Chitin Biosynthesis.- 1 Introduction.- 2 Structure of Chitin.- 3 The Enzymatic Synthesis of Chitin.- 4 Preparation of Chitin Synthase.- 5 Product Characterization of Chitin Synthase.- 6 Polymerization and Crystallization.- 7 Fungal Chitin Synthase Genes.- 8 Morphogenetic Roles of Chitin Synthases.- 9 Conclusions.- References.- Analysis of Plant-Substratum Adhesives.- 1 Introduction.- 2 Case Studies of Cell-Substratum Adhesion.- 3 Methods for the Analysis of Cell-Substratum Adhesives.- 4 Conclusions.- References.- Biochemical, Immunological and Molecular Analyses of Extensin.- 1 Introduction.- 2 Biochemical Characterization of Extensin.- 3 Immunological Detection of Extensin.- 4 Molecular Characterization of Extensin.- 5 Summary.- References.- Analysis of Pectin Structure by HPAEC-PAD.- 1 Introduction.- 2 Pectin HPLC Separations.- 3Pectin Analysis in Ripening Peach Fruit.- 4 Conclusions and Future Directions.- References.- Characterization of Oligosaccharides Derived from Plant Cell Wall Polysaccharides by On-Line High-Performance Anion-Exchange Chromatography Thermospray Mass Spectrometry.- 1 Introduction.- 2 Methods.- 3 HPAEC in Oligosaccharide Analysis.- 4 General Experimental Considerations Related to HPAEC-MS.- 5 Application of HPAEC-MS in Oligosaccharide Characterization.- 6 Conclusions and Perspectives.- References.- Analysis of Pectin Methyl Esterases.- 1 Introduction.- 2 Estimation and Detection of PME Activities.- 3 PME Localization.- 4 PME Extraction and Purification.- 5 PME Properties.- 6 Roles of PMEs.- 7 Conclusions and Perspectives.- References.- Probing the Subunit Composition and Topology of Plasma Membrane-Bound (1,3)-?-Glucan (Callose) Synthases.- 1 Introduction.- 2 Membrane Isolation, Enzyme Assay, and Solubilization.- 3 Callose Synthase Topology, Purification, and Subunit Composition.- 4 Biochemical Characterization of Integral Plant Plasma Membrane Proteins.- 5 Summary.- References.