RNP Particles, Splicing and Autoimmune Diseases de Johannes Schenkel

RNP Particles, Splicing and Autoimmune Diseases: Springer Lab Manuals

Editat de Johannes Schenkel

en Limba Engleză Paperback – 18 iul 2012

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Specificații

ISBN-13: 9783642489754
ISBN-10: 3642489753
Pagini: 236
Ilustrații: X, 225 p. 90 illus.
Dimensiuni: 155 x 235 x 12 mm
Greutate: 0.34 kg
Ediția:1998
Editura: Springer Berlin, Heidelberg
Colecția Springer
Seria Springer Lab Manuals

Locul publicării:Berlin, Heidelberg, Germany

Public țintă

Research

Cuprins

1 Isolation and Immunochemical Characterization of hnRNP Particles.- 2 The lnRNP Particle — A Naturally Assembled Complex of Pre-mRNA and Splicing Factors.- 3 Intrinsic Fluorescence Techniques for Studies on Protein-Protein and Protein-RNA Interactions in RNP Particles.- 4 Procedures for Three-Dimensional Reconstruction from Thin Sections with Electron Tomography.- 5 Purification and Electron Microscopy of Spliceosomal snRNPs.- 6 Detection of Autoantibodies to Extractable Cellular Antigens.- 7 Methods in Immunolocalization of Autoantigens.- 8 In vitro Splicing of Pre-mRNA in HeLa Extracts.- Abbreviations.

Textul de pe ultima copertă

The maturation of the precursors of mRNA to a functional transcript in eukaryotic cells is a complex, multistep process. It includes the formation of RNP complexes and the splicing procedure. Also, the presence of snRNA packaged as snRNP particles plays an important role in this process. In addition, snRNPs are involved in various autoimmune diseases in which autoantibodies are directed against components of these particles.
Relevant techniques to investigate RNA processing, splicing, protein-RNA complex formation and protein-RNA interactions as well as the use of autoantibodies in basic and clinical research are presented in this manual.

Caracteristici

A practical tool for anyone working at the lab bench Easy to follow step-by-step protocols Background information and full practical details

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