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Z-DNA: Methods and Protocols de Kyeong Kyu Kim

Z-DNA: Methods and Protocols: Methods in Molecular Biology, cartea 2651

Editat de Kyeong Kyu Kim, Vinod Kumar Subramani
en Limba Engleză Hardback – 10 mar 2023
This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z. 

Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
This is an open access book.
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Specificații

ISBN-13: 9781071630839
ISBN-10: 1071630830
Pagini: 332
Ilustrații: XIII, 332 p. 93 illus., 64 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.82 kg
Ediția:2023
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

The Origin of Left-Handed Poly[d(G-C)].- Characterization of Z-DNA Using Circular Dichroism.- Characterization of Z-DNA by Infrared Spectroscopy.- Crystallization of Z-DNA in Complex with Chemical and Z-DNA Binding Z-Alpha Protein.- NMR Titration Studies in Z-DNA Dynamics.- Single-Molecule Methods to Study Z-DNA Mechanics and Dynamics.- BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector.- Oligonucleotide Containing 8-Trifluoromethyl-2’-Deoxy-Guanosine as a Z-DNA Probe.- Chiroptical Properties of Z-DNA Using Ionic Porphyrins and Metalloporphyrins.- Construction of a Z-DNA Specific Recombinant Nuclease Zαα-FOK for Conformation Studies.- Human Heme Oxygenase-1 Promoter Activity is Mediated by Z-DNA Formation.- ChIP-Seq Strategy to Identify Z-DNA Forming Sequences in the Human Genome.- Detection of Z-DNA Structures in Supercoiled Genome.- Thermogenomic Analysis of Left-Handed Z-DNA Propensities in Genomes.- DeepZ: A Deep-Learning Approach for Z-DNA Prediction.- Methods to Study Z-DNA-Induced Genetic Instability.- Single-Molecule Visualization of B-Z Transition in DNA Origami Using High-Speed AFM.- Adoption of A-Z Junctions in RNAs by Binding of Zα Domains.- Detecting Z-RNA in Mammalian Cells.- Identification of ADAR1 p150 and p110 Associated Edit Sites.- Z-DNA and Z-RNA: Methods Past and Future.

Textul de pe ultima copertă

This detailed volume compiles key methods and techniques used to establish some of the structural and functional aspects of Z-form nucleic acids. Beginning with chapters on characterizing Z-DNA, the book continues by exploring structure determination techniques, gene- and genome-targeting methods and genome-wide approaches, Z-RNA study, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Z-DNA: Methods and Protocols serves as an ideal guide for students and researchers curious about this unique Z nucleic acid as an area of investigation, aiming to help the field grow, flourish, and unravel the hidden and novel roles of Z. 

Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts This book is open access, which means that you have free and unlimited access.