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Antibody Engineering Protocols: Methods in Molecular Biology, cartea 51

Editat de Sudhir Paul
en Limba Engleză Paperback – 13 iul 1995

Din seria Methods in Molecular Biology

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Specificații

ISBN-13: 9780896032750
ISBN-10: 0896032752
Pagini: 450
Ilustrații: XII, 450 p.
Dimensiuni: 155 x 235 x 28 mm
Greutate: 0.64 kg
Ediția:1995
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

SEQHUNT.- Molecular Modeling of Antibody-Combining Sites.- Structure and Properties of Human Immunoglobulin Light-Chain Dimers.- Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins.- Detection of Human Variable Gene Family Expression at the Single-Cell Level.- Purification of Reduced and Alkylated Antibody Subunits.- Murine Monoclonal Antibody Development.- Comparative Properties of Polyclonal and Monoclonal Antibodies.- Evaluation of Antibody Clonality.- Affinity Immunoblotting.- Epitope and Idiotope Mapping Using Monoclonal Antibodies.- Anti-Idiotypic Antibodies That Mimic Opioids.- Catalytic Antibodies.- Preparation and Assay of Acetylcholinesterase Antibody.- DNA Hydrolysis by Antibodies.- Screening Strategies for Catalytic Antitransition-State Analog Antibodies.- Expression of Chimeric Immunoglobulin Genes in Mammalian Cells.- Single-Chain Anti-DNA Fv.- Molecular Cloning of Antiground-State Proteolytic Antibody Fragments.- Cloning and Bacterial Expression of an Esterolytic sFv.- Site-Directed Mutagenesis of Antibody-Variable Regions.- Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation.- Chaperonins in Phage Display of Antibody Fragments.- Phage-Display Libraries of Murine and Human Antibody Fab Fragments.- Selection of Human Immunoglobulin Light Chains from a Phage-Display Library.- Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography.- Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening.- Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies.- Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies.- Radiolabeling of Antibodies for Therapy and Diagnosis.