Cantitate/Preț
Produs
Total produse
Vezi coșul
Antigen Processing: Methods and Protocols de Peter van Endert

Antigen Processing: Methods and Protocols: Methods in Molecular Biology, cartea 1988

Editat de Peter van Endert
en Limba Engleză Paperback – 14 aug 2020
The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.

Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (2) 73880 lei  38-44 zile
  Springer – 14 aug 2020 73880 lei  38-44 zile
  Humana Press Inc. – 23 aug 2016 115630 lei  6-8 săpt.
Hardback (2) 99891 lei  38-44 zile
  Springer – 30 mai 2019 99891 lei  38-44 zile
  Humana Press Inc. – 18 ian 2013 141791 lei  6-8 săpt.

Din seria Methods in Molecular Biology

  • 9% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 79159 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59856 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49679 lei
  • 20% Clinical Bioinformatics
    Preț: 88295 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 71733 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71911 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72883 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 73457 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 65228 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 100802 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 72221 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 89877 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 65342 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 64310 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 139083 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 73010 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 95589 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64937 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 72598 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 96831 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 72093 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39258 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 73370 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 94642 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86021 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 64166 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 103769 lei
  • 23% Computational Protein Design
    Preț: 88385 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79216 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42362 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42591 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59220 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103884 lei
  • 5% Investigations of Early Nutrition Effects on Long-Term Health: Methods and Applications
    Preț: 52415 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 208644 lei
  • 5% Epitope Mapping Protocols
    Preț: 127730 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78993 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133910 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 136679 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75266 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37489 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 137205 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 111057 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 138447 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 110593 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 94998 lei

Preț: 73880 lei

Preț vechi: 77768 lei
-5% Nou

Puncte Express: 1108
Preț estimativ în valută:
14143€ • 14546$ • 11733£

Carte tipărită la comandă

Livrare economică 15-21 februarie

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493994526
ISBN-10: 1493994522
Pagini: 458
Ilustrații: XV, 458 p. 79 illus., 45 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:2nd ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes.- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry.- Trimming of MHC Class I Ligands by ERAP Aminopeptidases.- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases.- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays.- In Vitro Studies of MHC Class I Peptide Loading and Exchange.- Measuring Synthesis and Degradation of MHC Class I Molecules.- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules.- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics.- Purification and Identification of Naturally Presented MHC Class I and II Ligands.- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs.- Quantitating MHC Class I Ligand Production and Presentation using TCR-LikeAntibodies.- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors.- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.- Evaluating CD8+ T Cell Responses In Vitro.- Recombinant Poxviruses: Versatile Tools for Immunological Assays.- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy.- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases.- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays.- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays.- In Vitro Digestion with Proteases Producing MHC Class II Ligands.- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors.- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading.- Studying MHC Class II Peptide Loading andEditing In Vitro.- Monitoring Antigen Processing for MHC Presentation via Macroautophagy.- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies.- Production of Antigen Specific Human CD4+ T Cell Lines and Clones.- Monitoring Dendritic Cell Activation and Maturation.- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes.- Analyzing Antigen Recognition by Natural Killer T Cells.

Textul de pe ultima copertă

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Recenzii

From the reviews:
“The book is divided into 42 chapters, each split into a short abstract, an introduction providing the relevant bibliographic overview, a detailed list of materials used in the protocol, a step-by-step description of the procedure, and a final section with notes. … Van Endert achieves what he set out to do – to provide an in-depth bench-side companion for antigen processing beginners and experts … . The book comes highly recommended.” (Clemens Hermann, Immunology News, February, 2014)
“The book is designed to be a benchside companion for researchers interested in studying antigen processing. It is intended for both beginners and experts studying antigen presentation.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)