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Arbuscular Mycorrhizal Fungi: Methods and Protocols de Nuria Ferrol

Arbuscular Mycorrhizal Fungi: Methods and Protocols: Methods in Molecular Biology, cartea 2146

Editat de Nuria Ferrol, Luisa Lanfranco
en Limba Engleză Paperback – 16 mai 2021
This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbiontsin agriculture more effectively.
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Specificații

ISBN-13: 9781071606056
ISBN-10: 1071606050
Pagini: 257
Ilustrații: XI, 257 p. 48 illus., 36 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:1st ed. 2020
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples.- The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants.- A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium.- Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization.- Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide.- Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity.- Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization.- Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi.- High Throughput DNA Sequence-Based Analysis of AMF Communities.- Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP).- Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencingin Arbuscular Mycorrhizal Fungi.- Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR.- Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis.- Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization.- Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast.- Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis.- Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae.- Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula.- Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi.

Textul de pe ultima copertă

This book compiles the most comprehensive collection of protocols currently used in arbuscular mycorrhizal (AM) fungal research. This experience-based collection includes methods for isolation, cultivation, detection, and quantification of AM fungi, as well as the use of metagenomics for community studies and experimental procedures for functional genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Arbuscular Mycorrhizal Fungi: Methods and Protocols serves as an ideal aid for researchers seeking to perform experiments that fill the gaps in our knowledge of the basic biology, functions, and ecology of AM fungi in the hope of deploying these powerful plant fungal symbiontsin agriculture more effectively.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts