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Intracellular Pathogens: Methods and Protocols de Aneesh Thakur

Intracellular Pathogens: Methods and Protocols: Methods in Molecular Biology, cartea 2813

Editat de Aneesh Thakur
en Limba Engleză Hardback – 19 iun 2024
This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intracellular Pathogens: Methods and Protocols serves as an ideal guide for researchers who work with intracellular pathogens and want to utilize or refine strategies to study, treat, or controlthem.
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Specificații

ISBN-13: 9781071638897
ISBN-10: 1071638890
Pagini: 385
Ilustrații: XII, 373 p. 60 illus., 58 illus. in color.
Dimensiuni: 178 x 254 x 27 mm
Greutate: 0.89 kg
Ediția:2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Intracellular Pathogens: Infection, Immunity, and Intervention.- Methods for Genomic Epidemiology of Bacterial Pathogens: Example Salmonella.- Phagocytosis.- Large Scale Culture and Plasmid Preparation Procedure for Low-Yield Bacmids Containing Full-Length SARS-CoV-2 cDNAs.- Metatranscriptomic RNA-Seq Data Analysis of Virus-Infected Host Cells.- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.- Quantitative Proteomics of the Intracellular Bacterial Pathogen Salmonella enterica Serovar Typhimurium.- Median Tissue Culture Infectious Dose 50 (TCID50) Assay to Determine Infectivity of Cytopathic Viruses.- Applications of Quantitative PCR (qPCR) in Studies of Virus-Host Interactions.- Establishing Air-Liquid Interface (ALI) Airway Culture Models for Infectious Disease Research.- Pig Acute Phase Proteins as Non-Antibody Systemic Biomarkers of Intracellular Infections.- Quantification of Mycobacterium tuberculosis Growth in Cell-Based Infection Assays by Time-Lapse Fluorescence Microscopy.- Intravital Microscopy for Imaging and Live Cell Tracking of Alveolar Macrophages in Real Time.- Recent Advancements in Radiopharmaceuticals for Infection Imaging.- Bacteriophages Isolation and Efficacy Testing.- Production, Isolation, and Detection of Poly IC in Extracellular Vesicles from U937 Cells.- In Silico Tools for Predicting Novel Epitopes.- In Situ Characterization of Human Follicular Helper CD4 T Cells.- Identification and Characterization of Antigen-Specific T-Cells in Viral Infections.- Evaluating Cationic Nanoparticles as Carriers of Antiviral Nucleic Acids.- RNA-Based Vaccines and Therapeutics Against Intracellular Pathogens.

Textul de pe ultima copertă

This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intracellular Pathogens: Methods and Protocols serves as an ideal guide for researchers who work with intracellular pathogens and want to utilize or refine strategies to study, treat, or controlthem.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts