Isothermal Titration Calorimetry in Enzymology: Techniques and Applications: Foundations and Frontiers in Enzymology
Editat de Anthony Mittermaier, Justin Di Tranien Limba Engleză Paperback – aug 2025
- Describes and instructs in cutting-edge ITC experiments and analysis techniques
- Covers both kinetics and thermodynamics-based ITC approaches
- Features illustrative case studies across a wide range of applications
- Includes chapter contributions from international experts in ITC
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Specificații
ISBN-13: 9780443218484
ISBN-10: 044321848X
Pagini: 472
Dimensiuni: 191 x 235 mm
Editura: ELSEVIER SCIENCE
Seria Foundations and Frontiers in Enzymology
ISBN-10: 044321848X
Pagini: 472
Dimensiuni: 191 x 235 mm
Editura: ELSEVIER SCIENCE
Seria Foundations and Frontiers in Enzymology
Cuprins
PART 1: ITC kinetics measurements 1. Enzyme kinetics by ITC 2. Measuring binding kinetics by ITC 3. Analysing ITC kinetic data with AFFinimeter software 4. In vivo enzyme kinetics by ITC 5. Characterizing enzyme kinetics by 2D-ITC 6. Measuring enzyme stability using ITC 7. Kinetics of immobilized enzymes 8. Enzyme kinetics in crowded solutions 9. Kinetics of nanozymes 10. Using ITC to characterize enzyme allostery 11. Signal amplification in ITC kinetics experiments 12. ITC kinetic studies of 6-phytase 13. Catalysis of biological substrates of nuclear metallohydrolases 14. Alpha-amylase activity and inhibition 15. Enzyme stability and activity of psycrophillic enzymes 16. Non-MM kinetics of peroxidases and application to industrial wastes 17. Kinetics of diphosphohydrolases PART 2: ITC thermodynamic measurements 18. Interpretation of ITC binding curves 19. Analysis of ITC binding data using SEDPHAT 20. ITC in drug discovery 21. ITC in the development of cancer therapeutics 22. Mechanisms of aminoglycoside resistance enzymes 23. Using ITC to characterize the interactions of natural products with biomolecules 24. ITC studies of metalloenzymes 25. Enzyme interactions with toxic metals 26. Thermodynamics of molecular machines 27. Thermodynamics of protein chaperones 28. Characterizing protein interactions in apoptosis by ITC 29. Using ITC to study ubiquitination 30. Thermodynamics of transition state analogs 31. Thermodynamics of recombinant enzymes in biotechnology 32. Ion Binding to Transport Proteins using ITC 33. Urease from Helicobacter pylori, lessons from ITC 34. Substrate recognition by Chitinases 35. ITC studies of glycoside hydrolases