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Transglutaminase: Fundamentals and Applications: Foundations and Frontiers in Enzymology

Editat de Yi Zhang, Benjamin K. Simpson
en Limba Engleză Paperback – 29 feb 2024
Transglutaminase: Fundamentals and Applications, a new volume in the Foundations and Frontiers in Enzymology series, stands as a comprehensive resource on this increasingly important class of enzymes. Following an introduction to the field, international experts discuss basic biology and structure – function relationships of transglutaminases, ongoing fundamental research, and new application areas, including food processing, material synthesis, biomedicine, disease treatment, and biotechnology. Approaches for sourcing transglutaminases from animals, plants, and microorganisms are considered, followed by chapters on applied uses in biomedicine, skin, bone biology, medicine, drug development, tissue engineering, seafood bioprocessing, dairy bioprocessing, synthesis of materials and polymers, and as an immobilization agent.

Research protocols are also provided, including research rationale, design, substrates, assays, and microassay steps for studying transglutaminases.

  • Brings together basic knowledge and applied research on transglutaminase biology
  • Considers a range of application areas, from biomedicine to drug development, tissue engineering, food processing, and materials synthesis
  • Includes instructive protocols for basic and applied transglutaminase research
  • Features chapter contributions from global leading experts in the field, across disciplines and applications
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Specificații

ISBN-13: 9780443191688
ISBN-10: 0443191689
Pagini: 382
Dimensiuni: 191 x 235 x 21 mm
Greutate: 0.75 kg
Editura: ELSEVIER SCIENCE
Seria Foundations and Frontiers in Enzymology


Cuprins

An introduction to transglutaminases
Yi Zhang, Srividya Parthasarathy, and Benjamin K. Simpson

Chapter 1 Transglutaminases from microorganisms
Limin Wang and Yi Wang
1. Introduction
2. Microbial sources of TGase
3. Biotechnological production of MTGase
4. Concluding remarks and outlook
References

Chapter 2 Transglutaminases from plant sources
Stefano Del Duca and Giampiero Cai
1. Introduction
2. Phylogenetic relationship and bioinformatic analyses
3. Distribution and physiological roles of TGases in plants
4. Conclusions
References

Chapter 3 Transglutaminases from animals
Jirawat Yongsawatdigul, Ali Hamzeh and Bung-orn Hemung
1. Introduction
2. Function and distribution
3. Conclusions
References

Chapter 4 Newly engineered transglutaminases
Han Liu, Ishita Ghosh, Corinne Nardin and Yi Zhang
1. Introduction
2. Methodology for engineering transglutaminases
3. Newly engineered microbial transglutaminases
4. Newly engineered animal transglutaminases
5. Regulations
References

Chapter 5 Transglutaminase 2 in neurological conditions
Thomas Delgado and Gail V.W. Johnson
1. Discovery of TG2 and its association with proteinopathies
2. TG2’s complex role in neuron survival
3. TG2 in CNS injury and neuroinflammation
4. Epigenetic regulation by TG2
5. Conclusions
References

Chapter 6 Transglutaminases in cardiovascular health and disease
Lakshmi Santhanam
Abbreviations
1. Introduction
2. Transglutaminases in the cardiovascular system
3. Transglutaminases in vascular diseases
4. Transglutaminases in cardiac disease
5. Transglutaminases in thromboembolism
6. Conclusions and future perspectives
References

Chapter 7 Microbial transglutaminase in drug development
Riko Nishioka, Ryo Sato, Kazuki Uchida, Rie Wakabayashi and Noriho Kamiya
1. Introduction
2. Protein modification using small molecule substrates
3. Preparation of proteineprotein conjugates
4. Protein modification using synthetic polymeric substrates
5. Introduction of hydrophobic molecules into proteins
6. Conclusion
Acknowledgments
References

Chapter 8 Transglutaminases in meat and seafood bioprocessing
Pınar Yerlikaya and Nalan Gokoglu
1. Transglutaminases in meat bioprocessing
2. Transglutaminases in seafood bioprocessing
3. Conclusion
References

Chapter 9 Transglutaminase in dairy processing
Ehab Romeih, Marek Kieliszek and Essam Hebishy
1. Introduction
2. MTGase applications in dairy processing
3. Conclusions and future remarks
References

Chapter 10 Transglutaminase in noodles, pastas, and baked products
Seyed Mohammad Taghi Gharibzahedi and Zeynep Altintas
1. Introduction
2. Noodle and TGase
3. Pasta and TGase
4. Bread and TGase
5. Other bakery products and TGase
6. Health and nutritional properties
7. Conclusions
Acknowledgments
References

Chapter 11 Transglutaminase in textile, wool, silk, and leather processing
Ning Zhang, Penghui Yang, Man Zhou, Qiang Wang, Song Liu and Jian Chen
1. Transglutaminase in wool processing
2. Transglutaminase in silk processing
3. Transglutaminase in leather processing
4. Conclusion
References

Chapter 12 Immobilization of transglutaminases and transglutaminase as immobilization agent
Katja Vasic, Zeljko Knez and Maja Leitgeb
1. Introduction
2. Enzymatic properties of TGase
3. Production and substrate preference of TGase
4. TGase immobilization
5. TGase as immobilizing agent in food and
biotechnology industries
6. Conclusions
Acknowledgments
References

Chapter 13 Industrial production of transglutaminase
Keiichi Yokoyama
1. Background to food innovation with transglutaminase
2. Feasibility study on protein modification by TGase
3. Search for a TGase for industrial applications
4. Developments for MTGase commercialization
5. MTGase in nature
6. Recombinant production of MTGase
7. MTGase variants with improved properties
8. Food safety and regulation
9. Concluding remarks
References
Further reading

Index