Neisseria meningitidis (Methods in Molecular Biology) de Kate L. Seib

Neisseria meningitidis: Methods and Protocols: Methods in Molecular Biology, cartea 1969

Kate L. Seib

Ian R. Peak

This volume provides methods to analyze the meningococcus and its interactions with biologically relevant host cells and sites, to interrogate the population structure and biology of the meningococcus that defines its capacity to cause disease, and to aid in vaccine development and surveillance. Many of these methods are applicable to the close relative, Neisseria gonorrhoeae, and several of the methods described can also be used in investigating host-pathogen interactions for a range of other organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Neisseria meningitidis: Methods and Protocols will allow for the use of these methods by more laboratories and foster collaboration andconsistency in investigations of this enigmatic and dangerous pathogen.

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Cuprins

An Overview of Neisseria meningitides.- Diagnosis of Meningococcal Infection Using Internally-Controlled Multiplex Real-Time PCR.- Investigating RNA-Protein Interactions in Neisseria meningitidis by RIP-Seq Analysis.- Genome Sequencing and Interrogation of Genome Databases: A Guide to Neisseria meningitidis Genomics.- Determination of Repeat Number and Expression States of Phase-Variable Loci through Next Generation Sequencing and Bioinformatic Analysis.- Screening DNA Repeat Tracts of Phase Variable Genes by Fragment Analysis.- Sample Preparation for Proteomic Analysis of Neisseria meningitides.- Investigation of Whole Cell Meningococcal Glycan Interactions Using High Throughput Glycobiology Techniques: Glycan Array and Surface Plasmon Resonance.- The Detroit 562 Pharyngeal Immortalized Cell Line Model for the Assessment of Infectivity of Pathogenic Neisseria sp..- In Vitro Models for Studying the Interaction of Neisseria meningitidis with Human Brain Endothelial Cells.- Modeling Neisseria meningitidis Infection in Mice: Methods and Logistical Considerations for Nasal Colonization and Invasive Disease.- Meningococcal Serogroup A, B, C, W, X, and Y Serum Bactericidal Antibody Assays.- Monoclonal Antibodies against the Capsular Polysaccharides A, C, Y, W, and X of Neisseria meningitides: A Platform for the Quality Control of Meningococcal Vaccines.- Meningococcal Antigen Typing System (MATS): A Tool to Estimate Global Coverage for 4CMenB, a Multicomponent Meningococcal B Vaccine.- Flow Cytometric Assays to Quantify fHbp Expression and Detect Serotype Specific Capsular Polysaccharides on Neisseria meningitides.- Assessment of fHbp Expression Level by Reverse Transcriptase Quantitative PCR and Promoter Sequence Analysis.

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Neisseria meningitidis: Methods and Protocols: Methods in Molecular Biology, cartea 1969

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