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Plant Protein Secretion: Methods and Protocols: Methods in Molecular Biology, cartea 2841

Editat de Liwen Jiang, Jinbo Shen, Caiji Gao, Xiangfeng Wang
en Limba Engleză Hardback – 16 sep 2024
This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.
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Specificații

ISBN-13: 9781071640586
ISBN-10: 1071640585
Pagini: 277
Ilustrații: Approx. 275 p. 55 illus., 48 illus. in color.
Dimensiuni: 178 x 254 x 20 mm
Greutate: 0.68 kg
Ediția:2nd ed. 2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Conventional and Unconventional Protein Secretion in Yeast and Animal Cells.- An Overview of Protein Secretion in Plant Cells.- Bioinformatic Analysis for Identifying Transcription Factors Involved in Protein Secretion and Vacuole Formation.- Using AlphaFold2 and Molecular Dynamics Simulation to Model Protein Recognition.- Proteomic Analysis of Secreted Vesicle Proteins from Plant Cells.- Extracellular Vesicle Isolation and Mass-Spectrometry-Based Proteomic Analysis in Arabidopsis thaliana.- Polysaccharides Analysis of Cell Wall Using Carbohydrate Gel Electrophoresis.- Acetyl Bromide Method for Total Lignin Content Determination in Plant Biomass.- Utilizing Plant Protein Secretion System for Pharmaceutical Protein Production.- In Vitro Reconstitution of Plant COPII Vesicles.- Identification of Neighboring Proteins of Endosomal Regulators by Using TurboID-Based Proximity Labeling.- Quantification of Protein Dynamics in Growing Pollen Tubes via Whole Cell Kymograph Analysis.- Polar Localization Analysis of Anionic Phospholipids and Their Binding Proteins in Arabidopsis Pollen Tubes.- Vacuum-Based Agroinfiltration for Transient Gene Expression in Arabidopsis Seedlings.- Pharmaceutical Analysis in the Plant Endomembrane System.- Observation of Vacuoles in Arabidopsis Developing Pollen Grain with Whole-Cell Electron Tomography.- Whole-Cell Electron Tomography Analysis of Vacuole Biogenesis.- Microscopy Analysis of Autophagosomal Structures in Plant Cells.- Electron Microscopy Analysis of Membraneless Condensates in Plant Cells.- High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.- Determination of the Cryo-EM Structure of ATG9 from Arabidopsis thaliana.- Genetic Screening of Factors in the Plant Protein Secretion.- Studying Autophagy and Senescence of Arabidopsis Stigmatic Cells.

Textul de pe ultima copertă

This volume explores the latest developments in protein secretion research in plants, as compared to yeast and mammalian systems. The chapters in this book present a diverse and thorough perspective of the field and cover topics such as bioinformatic analysis, proteomic studies, ultrastructural analysis, and genetic screening methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Plant Protein Secretion: Methods and Protocols, Second Edition is a valuable resource for researchers and students in the field of plant biology, and will inspire further advancements in our understanding of protein secretion in plant cells and beyond.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts