Protocols for Oligonucleotide Conjugates: Synthesis and Analytical Techniques: Methods in Molecular Biology, cartea 26

Protecting Groups in Oligonucleotide Synthesis.- Incorporation of Modified Bases into Oligonucleotides.- Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups.- Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups.- Postsynthesis Functionalization of Oligonucleotides.- Oligonucleotide-Enzyme Conjugates.- Oligonucleotides Containing Degenerate Bases.- Synthesis of [15N]-Labeled DNA Fragments.- Analysis and Purification of Synthetic Oligonucleotides by High-Performance Liquid Chromatography.- Sequence Analysis of Oligodeoxyribonucleotides.- Gel-Capillary Electrophoresis Analysis of Oligonucleotides.- Nuclear Magnetic Resonance Studies of Oligonucleotides.- Mass Spectrometry of Nucleotides and Oligonucleotides.- Extracting Thermodynamic Data From Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions.

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