Pulsed-Field Gel Electrophoresis: Protocols, Methods, and Theories: Methods in Molecular Biology, cartea 12

Electrophoretic Techniques.- Field-Inversion Gel Electrophoresis.- Resolving Multimegabase DNA Molecules Using Contour-Clamped Homogeneous Electric Fields (CHEF).- Separation and Size Determination of DNA over a 10–,200 kbp Range.- PFGE Using Double-Inhomogeneous Fields or Orthogonal Field-Alternating Gel Electrophoresis (OFAGE).- Transverse Alternating-Field Electrophoresis.- Rotating Field Gel Electrophoresis (ROFE).- Preparation, Manipulation, and Pulse Strategy for One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).- Auxiliary Methods.- Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel Electrophoresis.- Encapsulation of Cells in Agarose Beads.- Preparation and Separation of Intact Chromosomes of Vertebrates by One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).- Isolation of Plant DNA for Pulsed-Field Gel Electrophoresis.- Methyltransferases as Tools to Alter the Specificity of Restriction Endonucleases.- Desktop Digital Imaging.- Biological Applications.- Bacterial Genome Mapping by Two-Dimensional Pulsed-Field Gel Electrophoresis (2D-PFGE).- Protozoan Genomes.- Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis.- Analysis of Yeast Artificial Chromosome Clones.- Strategies for Mapping Large Regions of Mammalian Genomes.- Two-Dimensional DNA Electrophoresis (2D-DE) for Mammalian DNA.- Pulsed-Field and Two-Dimensional Gel Electrophoresis of Long Arrays of Tandemly Repeated DNA.- Construction of Lambda Libraries from Large PFGE Fragments.- In-Gel Detection of DNA.- Theories and Observations.- Theories of Pulsed-Field Gel Electrophoresis.- The Dynamics of DNA Gel Electrophoresis.- Orientation of the Agarose Matrix by Pulsed Electric Fields.- Reptation Theory ofPulsed Electrophoresis and Trapping Electrophoresis.- Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE).

...an excellent reference and experimental manual...a must for every biochemistry and molecular biology library.-Journal of the American Chemical Society