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Recombinant Protein Production in Yeast de Brigitte Gasser

Recombinant Protein Production in Yeast: Methods in Molecular Biology, cartea 1923

Editat de Brigitte Gasser, Diethard Mattanovich
en Limba Engleză Hardback – 9 feb 2019
This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications. 
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Specificații

ISBN-13: 9781493990238
ISBN-10: 1493990233
Pagini: 350
Ilustrații: XII, 431 p. 60 illus., 46 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.98 kg
Ediția:1st ed. 2019
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Established and Upcoming Yeast Expression Systems.- Detection and Elimination of Cellular Bottlenecks in Protein Producing Yeasts.- Pichia pastoris Promoters.- Selection of the Optimal Yeast Host for the Synthesis Recombinant Enzymes.- Screening and Selection of Production Strains: Secretory Protein Expression and Analysis in Hansenula polymorpha.- Selection of Heterologous Protein Producing Strains in Yarrowia lipolytica.- High-throughput Screening and Selection of Pichia pastoris Strains.- Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics Roslyn M. Bill, Annegret Ulke.- CRISPR/Cas9-mediated Homology Directed Genome Editing in Pichia pastori.- Tailoring N-glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae.- Improving the Production of Cofactor Containing Proteins: Production of Human Hemoglobin in Yeast.- Membrane Protein Production in Yeast: Modification of Yeast Membranes for Human Membrane Protein Production.- Yeast Surface Display and Cell Sorting of Antigen-binding Fc Fragments.- Guidelines for Small-scale Production and Purification of Hepatitis B surface Antigen Virus-like Particles from Recombinant Pichia pastoris.- Efficient Development of a Mixed Feed Process for Pichia pastoris.- Primary Recovery of Yeast Culture Supernatant for Recombinant Protein Purification.- Purification of Recombinant glyco-proteins from Pichia pastoris Culture Supernatants.- LC-MS analysis of (glyco)-proteins of Pichia pastoris.- Assessing the Quality of Recombinant Products Made in Yeast.- Quantification of Recombinant Products In Yeast. 


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Textul de pe ultima copertă

This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications. 

Caracteristici

Include cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts