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RNA Vaccines: Methods and Protocols de Thomas Kramps

RNA Vaccines: Methods and Protocols: Methods in Molecular Biology, cartea 2786

Editat de Thomas Kramps
en Limba Engleză Hardback – 31 mai 2024
This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, RNA Vaccines: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this important and developing field.  

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Specificații

ISBN-13: 9781071637692
ISBN-10: 107163769X
Ilustrații: XII, 389 p. 57 illus., 49 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.92 kg
Ediția:2nd ed. 2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Introduction to RNA Vaccines Post COVID-19.- Self-Replicating RNA Derived from the Genomes of Positive Strand RNA Viruses.- Negative Strand RNA Virus Vectored Vaccines.- New Generation Self-Replicating RNA Vaccines Derived from Pestivirus Genome.- Trans-Amplifying RNA Vaccines Against Infectious Diseases: A Comparison with Non-Replicating and Self-Amplifying RNA.- Generation and Characterization of In Vitro Transcribed mRNA.- Production and Evaluation of Nucleoside-Modified mRNA Vaccines for Infectious Diseases.- Sequence-Optimized mRNA Vaccines Against Infectious Disease.- Design and Synthesis of Circular RNA Expression Vectors.- Electroporation of mRNA as a Universal Technology Platform to Transfect a Variety of Primary Cells with Antigens and Functional Proteins.- A Basic Method for Formulating mRNA-Lipid Nanoparticle Vaccines in the Lab.- Messenger RNA Lipid-Based Nanoparticles: Optimization of Formulations in the Lab.- Plant Expression of Trans-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Update.- Producing Plasmid DNA Template for Clinical Grade RNA Vaccine Manufacture.- Regulatory Considerations for Producing mRNA Vaccines for Clinical Trials.- Quality by Digital Design for Developing Platform RNA Vaccine Manufacturing Processes.- Nonclinical Safety Testing of RNA Vaccines.

Textul de pe ultima copertă

This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, RNA Vaccines: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this important and developing field.  

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts