Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods: Methods in Molecular Biology, cartea 50
Autor Justin P. Clappen Limba Engleză Paperback – 26 oct 1995
Din seria Methods in Molecular Biology
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Specificații
ISBN-13: 9780896033238
ISBN-10: 0896033236
Pagini: 416
Ilustrații: XI, 416 p.
Dimensiuni: 152 x 229 x 33 mm
Greutate: 0.71 kg
Ediția:1996
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
ISBN-10: 0896033236
Pagini: 416
Ilustrații: XI, 416 p.
Dimensiuni: 152 x 229 x 33 mm
Greutate: 0.71 kg
Ediția:1996
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology
Locul publicării:Totowa, NJ, United States
Public țintă
ResearchCuprins
Isolation and Purification of Plant Nucleic Acids.- Isolation and Purification of Insect DNA.- Isolation and Purification of Vertebrate DNAs.- Statistical Analysis of Arbitrarily Primed PCR Patterns in Molecular Taxonomic Studies.- Molecular Identification of Phytopathogenic Viruses.- Improved PCR Methods for Identification of Phytopathogenic Viruses.- PCR Detection of HIV.- The Use of Consensus PCR and Direct Sequence Analysis for the Identification of HPV.- The Identification of Dengue Virus Using PCR.- The Design and Application of Ribosomal RNA-Targeted, Fluorescent Oligonucleotide Probes for the Identification of Endosymbionts in Protozoa.- Subtraction Hybridization for the Isolation of Strain-Specific Rhizobium DNA Probes.- The Use of RAPD for Generating Specific DNA Probes for Microorganisms.- ITS-RFLP Matching for Identification of Fungi.- Specific PCR Primers for the Identification of Endomycorrhizal Fungi.- Single-Strand Conformational Polymorphism (SSCP) for the Identification of Endomycorrhizal Fungi.- The Use of RAPD for Isolate Identification of Arbuscular Mycorrhizal Fungi.- Establishing Relationships Between Closely Related Species Using Total Genomic DNA as a Probe.- Detection and Characterization of Leishmania Parasites by DNA-Based Methods.- The Molecular Identification of Trypanosomes.- Detection and Identification of the Four Malaria Parasite Species Infecting Humans by PCR Amplification.- The Use of Degenerate Primers in Conjunction with Strain and Species Oligonucleotides to Classify Onchocerca volvulus.- The Use of Synthetic DNA Probes for the Field Identification of Members of the Anopheles gambiae Complex.- PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Anopheles gambiae Complex.- Restriction Fragment Length Polymorphism (RFLP) Analyses.- Differential Screening for the Isolation of Species-Specific Sequences in the Anopheles farauti Complex.- RAPD-PCR with Parasitic Hymenoptera.- The Use of Selective Enrichment for the Isolation of Species-Specific DNA Probes for Insects.
Recenzii
...a wonderful compilation of modern PCR-type species identification methods. Methods are clerly presented with illustrations as needed. This manual is a must for those using or considering the use of PCR as a mechanism of species identification.-SIM News