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Transmembrane β-Barrel Proteins: Methods and Protocols de Raffaele Ieva

Transmembrane β-Barrel Proteins: Methods and Protocols : Methods in Molecular Biology, cartea 2778

Editat de Raffaele Ieva
en Limba Engleză Hardback – 14 mar 2024
This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane β-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Transmembrane β-Barrel Proteins: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of these vital membrane-spanning proteins.
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Specificații

ISBN-13: 9781071637333
ISBN-10: 1071637339
Ilustrații: XI, 386 p. 75 illus., 54 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.91 kg
Ediția:2024
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

The Name is Barrel, β-Barrel.- Recombinant Expression and Overproduction of Transmembrane b-Barrel Proteins.- Bacterial Envelope Fractionation.- Fluorescent Labeling of Outer Membrane Proteins Using the SpyCatcher-SpyTag System.- In Vitro Reconstruction of Bacterial β-Barrel Membrane Protein Assembly Using E. coli Microsomal (Mid-Density) Membrane.- Examining Protein Translocation by β-Barrel Membrane Proteins Using Reconstituted Proteoliposomes.- In Vivo Disulfide-Bond Crosslinking to Study β-Barrel Membrane Protein Interactions, Dynamicity, and Folding Intermediates.- Site-Specific Photocrosslinking to Investigate Toxin Delivery Mediated by the Bacterial β-Barrel Assembly Machine.- Analysis of Transmembrane β-Barrel Proteins by Native and Semi-Native Polyacrylamide Gel Electrophoresis.- Affinity Purification of Membrane β-Barrel Proteins via Biotin-Tagged Peptidiscs.- Monitoring the Interaction of the Peptidoglycan with the Bacterial β-Barrel Assembly Machinery.- MitoLuc:A Luminescence-Based Assay to Study Real-Time Protein Import into Mitochondria.- Modular Assembly of Mitochondrial β-Barrel Proteins.- Tracking the Activity and Position of Mitochondrial β-Barrel Proteins.- Conformational Heterogeneity of β-Barrel Membrane Proteins Observed In Situ Using Orthogonal Spin Labels and Pulsed ESR Spectroscopy.- Characterization of β-Barrel Outer Membrane Proteins and Their Interactions with Chaperones by Chemical-Crosslinking Mass Spectrometry.- Dissecting the Organization of a β-Barrel Assembly Machinery (BAM) Complex by Neutron Reflectometry.- Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.- Recent Advances in Modeling Membrane β-Barrel Proteins Using Molecular Dynamics Simulations: From Their Lipid Environments to Their Assemblies.- Structural Modeling of T9SS Outer Membrane Proteins and Their Complexes.- Rationale in Custom Design of Transmembrane β-Barrel Pores.- Stress-Based Screening for Compounds that Inhibit β-Barrel Outer Membrane Protein Assembly in Gram-Negative Bacteria.

Textul de pe ultima copertă

This detailed volume explores experimental strategies and protocols for the expression, assembly, characterization, and exploitation of transmembrane β-barrel proteins. Beginning with methodologies to study their assembly, the book continues with protocols for characterizing the landscape of transmembrane β-barrel protein interactions with other cellular factors, dissecting processes of protein transport in bacteria and mitochondria, examining structural characterization, determination, and prediction, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Transmembrane β-Barrel Proteins: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of these vital membrane-spanning proteins.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts