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Advanced Fluorescence Microscopy: Methods and Protocols de Peter J. Verveer

Advanced Fluorescence Microscopy: Methods and Protocols: Methods in Molecular Biology, cartea 1251

Editat de Peter J. Verveer
en Limba Engleză Hardback – 13 noi 2014
This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
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Specificații

ISBN-13: 9781493920792
ISBN-10: 1493920790
Pagini: 275
Ilustrații: XI, 294 p. 86 illus., 65 illus. in color.
Dimensiuni: 178 x 254 x 20 mm
Greutate: 0.75 kg
Ediția:2015
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Public țintă

Professional/practitioner

Cuprins

The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications.- Two-Photon Excitation Microscopy And Its Applications In Neuroscience.- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy.- Fluorescence Microscopy Based RNA Interference Screening.- Fluorescence Resonance Energy Transfer Microscopy (FRET).- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy.- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation.- Fluorescence Correlation Spectroscopy.- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules.- Practical Structured-Illumination Microscopy.- 4Pi Microscopy of the Nuclear Pore Complex.- Application Of STED Microscopy To Cell Biology Questions.- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes.- Direct Stochastic Optical Reconstruction Microscopy (dSTORM).- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.

Textul de pe ultima copertă

This volume provides an overview of advanced  fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems.  Written in the highly successful Methods in Molecular Biology  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Authoritative and cutting-edge,  Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras