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Affinity Chromatography: Methods and Protocols: Methods in Molecular Biology, cartea 1286

Editat de Senta Reichelt
en Limba Engleză Paperback – 22 oct 2016
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
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Specificații

ISBN-13: 9781493955848
ISBN-10: 1493955845
Pagini: 341
Ilustrații: XIII, 341 p. 78 illus., 37 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:Softcover reprint of the original 3rd ed. 2015
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Affinity Chromatography: A Historical.- Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography.- One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography.- Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes.- Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand.- Cell Affinity Separations on Microfluidic Devices.- Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins.- The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins.- Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins.- Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins.- Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products.- Mixed-bed Affinity Chromatography: Principles And Methods.- Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules.- Introduction to Macroporous Cryogels.- Cryogels with Affinity Ligands as Tools in Protein Purification.- Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography.- Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption.- A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum.-  Molecularly Imprinted Cryogels for Human Serum Albumin Depletion.- Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties.- Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin.- Accurateprotein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples.- Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis.- Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography.- Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies.

Textul de pe ultima copertă

The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols,  molecular modelling,  and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
 
Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is  designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step details essential for reproducible results Contains key notes and implementation advice from the experts