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Alternative Splicing: Methods and Protocols de Peter Scheiffele

Alternative Splicing: Methods and Protocols: Methods in Molecular Biology, cartea 2537

Editat de Peter Scheiffele, Oriane Mauger
en Limba Engleză Paperback – 28 iul 2023
This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
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Specificații

ISBN-13: 9781071625231
ISBN-10: 1071625233
Pagini: 354
Ilustrații: XI, 354 p. 61 illus., 57 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.64 kg
Ediția:1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Alternative Splicing in Human Biology and Disease.- Targeting Alternative Splicing for Therapeutic Interventions.- An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions.- FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis.- Quantitative Measurement of Alternatively Spliced RNA Isoform Levels.- Analysis of Splicing Regulation by Third-Generation Sequencing.- Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework.- Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data.- Functional Annotation of Custom Transcriptomes.- Design, Labeling, and Application of Probes for RNA smFISH.- BaseScope™ Approach to Visualize Alternative Splice Variants in Tissue.- In Situ Imaging of mRNA Splicing Variants by SpliceRCA.- Two-Color Fluorescent Reporters for Analysis of Alternative Splicing.- Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry.- Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy.- Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention.- Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins.- Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo.- Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.

Textul de pe ultima copertă

This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts