Inaddition to updating important core techniques this third edition presents newchapters on assessing leukocyte involvement in angiogenesis, lymphatic cell andpericyte isolation techniques, spheroid and arterial ring based in vitroassays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,Third Edition also guides readers through new protocols on chorioallantoicmembrane models, corneal pocket assays to assess angio- and lymphangiogenesis,models of muscle angiogenesis, and use of zebrafish embryos to study vascularangiogenesis and senescence. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Authoritativeand practical, Angiogenesis Protocols, Third Edition provides not only apractical handbook for key techniques, but also an informative and enjoyableread for all those interested, no matter how directly, in angiogenesis.