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Antisense RNA Design, Delivery, and Analysis de Virginia Arechavala-Gomeza

Antisense RNA Design, Delivery, and Analysis: Methods in Molecular Biology, cartea 2434

Editat de Virginia Arechavala-Gomeza, Alejandro Garanto
en Limba Engleză Paperback – 26 feb 2022
This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the workof newcomers to this vibrant and expanding field. 

This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics).  DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.
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Specificații

ISBN-13: 9781071620120
ISBN-10: 1071620126
Pagini: 410
Ilustrații: XVII, 422 p. 69 illus., 59 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.76 kg
Ediția:1st ed. 2022
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Introduction and History of the Chemistry of Nucleic Acids Therapeutics.- Antisense RNA Therapeutics: A Brief Overview.- Design of Bifunctional Antisense Oligonucleotides for Exon Inclusion.- Design and Delivery of SINEUP: A New Modular Tool to Increase Protein Translation.- How to Design U1 snRNA Molecules for Splicing Rescue.- Conjugation of Nucleic Acids and Drugs to Gold Nanoparticles.- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay.- Generation of Protein-Phosphorodiamidate Morpholino Oligomer Conjugates for Efficient Cellular Delivery via Anthrax Protective Antigen.- Development and Use of Cellular Systems to Assess and Correct Splicing Defects.- Modeling Splicing Variants Amenable to Antisense Therapy by Use of CRISPR-Cas9-Based Gene Editing in HepG2 Cells.- In Vitro Models for the Evaluation of Antisense Oligonucleotides in Skin.- In Vitro Delivery of PMOs in Myoblasts by Electroporation.- Rapid Determination of MBNL1 Protein Levels by Quantitative Dot Blot for Evaluation of Antisense Oligonucleotides in Myotonic Dystrophy Myoblasts.- Evaluation of Exon Skipping and Dystrophin Restoration in In Vitro Models of Duchenne Muscular Dystrophy.- Generation of Human iPSC-Derived Myotubes to Investigate RNA-Based Therapies In Vitro.- Eye on a Dish Models to Evaluate Splicing Modulation.- Establishment of In Vitro Brain Models for AON Delivery.- Considerations for Generating Humanized Mouse Models to Test Efficacy of Antisense Oligonucleotides.- Generation of Humanized Zebrafish Models for the In Vivo Assessment of Antisense Oligonucleotide-Based Splice Modulation Therapies.- Use of Small Animal Models for Duchenne and Parameters to Assess Efficiency upon Antisense Treatment.- In Vivo Models for the Evaluation of Antisense Oligonucleotides in Skin.- Delivery of Antisense Oligonucleotides to the Mouse Retina.- Delivery of Antisense Oligonucleotides to the Mouse Brain by Intracerebroventricular Injections.- Intrathecal Delivery of Therapeutic Oligonucleotides for Potent Modulation of Gene Expression in the Central Nervous System.- Preclinical Safety Assessment of Therapeutic Oligonucleotides.- Preclinical Evaluation of the Renal Toxicity of Oligonucleotide Therapeutics in Mice.- Protocol for Isolation and Culture of Mouse Hepatocytes (HCs), Kupffer Cells (KCs), and Liver Sinusoidal Endothelial Cells (LSECs) in Analyses of Hepatic Drug Distribution.- Patent Considerations When Embarking on New Antisense Drug Programs.

Textul de pe ultima copertă

This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention. Beginning with a section on the design of antisense technology and their delivery, the book continues by covering model systems developed to evaluate efficacy, both in vivo and in vitro, as well as methods to evaluate preclinically the toxicity associated with these new potential drugs, and intellectual property considerations. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antisense RNA Design, Delivery, and Analysis provides basic knowledge and a large collection of methods to facilitate the workof newcomers to this vibrant and expanding field. 

This book was conceived thanks to the network DARTER (Delivery of Antisense RNA Therapeutics).  DARTER is funded by the EU Cooperation of Science and Technology (COST), which aims to enhance interaction and collaborations between researchers in Europe and other countries.

Caracteristici

This book is open access, which means that you have free and unlimited access Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts