Cantitate/Preț
Produs

Argonaute Proteins: Methods and Protocols: Methods in Molecular Biology, cartea 725

Editat de Tom C. Hobman, Thomas F. Duchaine
en Limba Engleză Hardback – 2 mai 2011
With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 87369 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 87369 lei  6-8 săpt.
Hardback (1) 64152 lei  6-8 săpt.
  Humana Press Inc. – 2 mai 2011 64152 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 64152 lei

Preț vechi: 75473 lei
-15% Nou

Puncte Express: 962

Preț estimativ în valută:
12281 12766$ 10183£

Carte tipărită la comandă

Livrare economică 05-19 februarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781617790454
ISBN-10: 1617790451
Pagini: 315
Ilustrații: XII, 344 p.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.82 kg
Ediția:2011
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Purification of Native Argonaute Complexes from the Fission Yeast Schizosaccharomyces pombe.- Chromatin Immuno-Precipitation in Fission Yeast.- Biochemical Analyses of Endogenous Argonaute Complexes Immunopurified with Anti-Argonaute Monoclonal Antibodies.- Mapping of Ago2-GW182 Functional Interactions.- Continuous Density Gradients to Study Argonaute and GW182 Complexes Associated with the Endocytic Pathway.- In vitro RISC Cleavage Assay.- Native Gel Analysis for RISC Assembly.- Purification and Assembly of Human Argonaute, Dicer, and TRBP Complexes.- Detection of Human Dicer and Argonaute 2 Catalytic Activity.- Imaging the Cellular Dynamics of Drosophila Argonaute Proteins.- Live Cell Imaging of Argonaute Proteins in Mammalian Cells.- Reporter-Based Assays for Analyzing RNA Interference in Mammalian Cells.- Artificial Tethering of Argonaute Proteins for Studying their Role in Translational Repression of Target mRNAs.- An Efficient System For Let-7 MicroRNA- And GW182 Protein-Mediated Deadenylation In vitro.- Cell-Free microRNA-Mediated Translation Repression in C. elegans.- Argonaute Pull-Down and RISC Analysis Using 2'-O-Methylated Oligonucleotides Affinity Matrices.- Cloning Argonaute-Associated Small RNAs from C. elegans.- Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs.- Generation of an Inducible Mouse ES Cell Line Deficient for Argonaute Proteins.- Whole Cell Proteome Regulation by microRNAs Captured in a Pulsed SILAC Mass Spectrometry Approach.

Recenzii

From the reviews:
“It can be fully appreciated the high quality of the book, the so well organized and sequentially logic presentation of the chapters. … giving a very update and detailed presentation of the entire Argonaute family members, their evolutionary conservation in Eukaryotes, mechanisms of  action, methods to study  in vitro and in vivo … . each biologist and physician, either involved in research or in clinical application will find irreplaceable this book. Hope the reader will get his own copy of this exciting book.” (Manuela Monti, European Journal of Histochemistry, July, 2011)

Textul de pe ultima copertă

With the rapid proliferation of RNAi applications in basic and clinical sciences, the challenge has now become understanding how components of RNAi machinery function together in a regulated manner.  Argonaute proteins are the central effectors of RNAi and are highly conserved among eukaryotes and some archaebacteria. These RNA-binding proteins use small guide RNAs to silence expression of genes at the mRNA and DNA levels.  In Argonaute Proteins: Methods and Protocols, expert researchers in this burgeoning field provide detailed, up-to-date methods to study Argonaute protein functions and interactions in a wide variety of cell types ranging from yeast to mammalian systems, as well as in vitro.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
Practical and authoritative, Argonaute Proteins: Methods and Protocols serves as a vital reference for both experienced and novice scientists approaching the vast complexities of RNAi research.

Caracteristici

Aids researchers in examining these versatile proteins in a wide variety of cell types Features practical, step-by-step protocols which open the door to future progress in the field of molecular therapeutics Contains expert tips and vital implementation advice to ensure success