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Axon Degeneration: Methods and Protocols de Elisabetta Babetto

Axon Degeneration: Methods and Protocols: Methods in Molecular Biology, cartea 2143

Editat de Elisabetta Babetto
en Limba Engleză Paperback – 11 iun 2020
This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.

Chapters 2, 3, and 20 are availableOpen Access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Specificații

ISBN-13: 9781071605875
ISBN-10: 1071605879
Ilustrații: XI, 340 p. 80 illus., 58 illus. in color.
Dimensiuni: 178 x 254 mm
Ediția:1st ed. 2020
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Axon Degeneration: Which Method to Choose?.- Axon Degeneration Assays in Superior Cervical Ganglion Explant Cultures.- Microinjection of Superior Cervical Ganglion Neurons for Studying Axon Degeneration.- Assessing Axonal Degeneration in Embryonic Dorsal Root Ganglion Neurons In Vitro.- Viral Transduction of DRG Neurons.- Planning and Analysis of Axon Degeneration Screening Experiments.- A Microfluidic Culture Platform to Assess Axon Degeneration.- A Schwann Cell-Neuron Co-Culture System to Study Neuron-Glia Interaction during Axon Degeneration.- Establishing Myelinating Co-Cultures Using Human iPSC-Derived Sensory Neurons to Investigate Axonal Degeneration and Demyelination.- Preparation of Organotypic Hippocampal Slice Cultures for the Study of CNS Disease and Damage.- Organotypic Culture Assay for Neuromuscular Synaptic Degeneration and Function.- Intracellular Recordings of Postsynaptic Voltage Responses at the Drosophila Neuromuscular Junction.- Ex Vivo Studies of OpticNerve Axon Electrophysiology.- Ex Vivo Analysis of Axonal Degeneration Using Sciatic and Optic Nerve Preparations.- Measuring Bioenergetic Signatures of Peripheral Nerve Segments by Extracellular Flux Analysis.- Detection of Neutrophils in the Sciatic Nerve Following Peripheral Nerve Injury.- Whole-Tissue Immunolabeling and 3D Fluorescence Imaging to Visualize Axon Degeneration in the Intact, Unsectioned Mouse Tissues.- Transmission Electron Microscopy and Morphometry of the CNS White Matter.- In Vivo Calcium Imaging During Axon Degeneration in Zebrafish.- In Vivo Imaging of Anterograde and Retrograde Axonal Transport in Rodent Peripheral Nerves.- In Vivo Visualization of Moving Synaptic Cargo Complexes within Drosophila Larval Segmental Axons.- A Chemotherapy-Induced Peripheral Neuropathy Model in Drosophila melanogaster.- Models of Axon Degeneration in Drosophila Larvae.- In Vivo Analysis of Glial Immune Responses to Axon Degeneration in Drosophila melanogaster.

Textul de pe ultima copertă

This book is a collection of classical as well as innovative methods used to investigate axon degeneration with a particular focus on addressing the common challenges encountered while performing these procedures. Particular attention is devoted to the study of axon loss in several model organisms, as each poses unique challenges and provides powerful advantages. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Axon Degeneration: Methods and Protocols is an ideal guide for facilitating the application and further development of these protocols, which will help the scientific community tackle important questions regarding axon degeneration.

Chapters 2, 3, and 20 are availableOpen Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Caracteristici

Includes cutting-edge techniques Provides step-by-step detail essential for reproducible results Contains key implementation advice from the experts