Cantitate/Preț
Produs
Total produse
Vezi coșul
Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing de Shaying Zhao

Bacterial Artificial Chromosomes: Volume 1: Library Construction, Physical Mapping, and Sequencing: Methods in Molecular Biology, cartea 255

Editat de Shaying Zhao, Marvin Stodolsky
en Limba Engleză Hardback – 4 mar 2004
For both volumes:

Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (2) 62505 lei  43-57 zile
  Humana Press Inc. – 10 noi 2010 62505 lei  43-57 zile
  Humana Press Inc. – 10 noi 2010 62699 lei  43-57 zile
Hardback (2) 63174 lei  43-57 zile
  Humana Press Inc. – 4 mar 2004 63174 lei  43-57 zile
  Humana Press Inc. – 4 mar 2004 63395 lei  43-57 zile

Din seria Methods in Molecular Biology

  • 70% Nucleic Acid and Peptide Aptamers: Methods and Protocols
    Preț: 26096 lei
  • 23% Pheromone Signaling: Methods and Protocols
    Preț: 59855 lei
  • 19% Plant-Pathogen Interactions: Methods and Protocols
    Preț: 57771 lei
  • Engineering and Analyzing Multicellular Systems: Methods and Protocols
    Preț: 49678 lei
  • 20% Clinical Bioinformatics
    Preț: 88293 lei
  • Mitochondrial Regulation: Methods and Protocols
    Preț: 25204 lei
  • 5% Multiple Sclerosis: Methods and Protocols
    Preț: 70873 lei
  • 5% Bacterial Persistence: Methods and Protocols
    Preț: 71049 lei
  • 5% DNA-Protein Interactions: Principles and Protocols
    Preț: 72009 lei
  • 5% Methods in Mouse Atherosclerosis
    Preț: 72575 lei
  • 15% Cell Cycle Oscillators: Methods and Protocols
    Preț: 64446 lei
  • 18% TGF-β Signaling: Methods and Protocols
    Preț: 99590 lei
  • 5% Whole Genome Amplification: Methods and Protocols
    Preț: 71354 lei
  • 18% Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization
    Preț: 88795 lei
  • 15% Phospho-Proteomics: Methods and Protocols
    Preț: 64558 lei
  • 15% Candida Species: Methods and Protocols
    Preț: 63539 lei
  • 18% Induced Pluripotent Stem (iPS) Cells: Methods and Protocols
    Preț: 137406 lei
  • 5% Post-Transcriptional Gene Regulation
    Preț: 72134 lei
  • 20% In Vitro Embryogenesis in Higher Plants
    Preț: 82163 lei
  • 18% Yeast Functional Genomics: Methods and Protocols
    Preț: 94440 lei
  • 15% Statistical Analysis in Proteomics
    Preț: 64158 lei
  • 5% SiRNA Delivery Methods: Methods and Protocols
    Preț: 71727 lei
  • 18% Estrogen Receptors: Methods and Protocols
    Preț: 95666 lei
  • 5% Suppression and Regulation of Immune Responses: Methods and Protocols, Volume II
    Preț: 71229 lei
  • Plant Bioinformatics: Methods and Protocols
    Preț: 39257 lei
  • 5% Breast Cancer: Methods and Protocols
    Preț: 72490 lei
  • 18% Endocannabinoid Signaling: Methods and Protocols
    Preț: 93503 lei
  • 23% Zebrafish: Methods and Protocols
    Preț: 86019 lei
  • 15% Activity-Based Proteomics: Methods and Protocols
    Preț: 63395 lei
  • 5% Drug Target miRNA: Methods and Protocols
    Preț: 102521 lei
  • SH2 Domains: Methods and Protocols
    Preț: 79215 lei
  • Cholesterol Homeostasis: Methods and Protocols
    Preț: 42361 lei
  • 5% The Immune Synapse: Methods and Protocols
    Preț: 42590 lei
  • Organ Regeneration: 3D Stem Cell Culture & Manipulation
    Preț: 59219 lei
  • 5% Innate Antiviral Immunity: Methods and Protocols
    Preț: 34562 lei
  • 5% Synthetic Metabolic Pathways: Methods and Protocols
    Preț: 37270 lei
  • 19% Protein Engineering: Methods and Protocols
    Preț: 49188 lei
  • 5% Innate Immune Activation: Methods and Protocols
    Preț: 103882 lei
  • 18% Planarian Regeneration: Methods and Protocols
    Preț: 206129 lei
  • 5% Epitope Mapping Protocols
    Preț: 126192 lei
  • Tissue Proteomics: Methods and Protocols
    Preț: 78992 lei
  • 5% Genetic Epidemiology: Methods and Protocols
    Preț: 133907 lei
  • 18% Plant Chemical Genomics: Methods and Protocols
    Preț: 135031 lei
  • 5% Lung Innate Immunity and Inflammation: Methods and Protocols
    Preț: 75265 lei
  • 5% Rational Drug Design: Methods and Protocols
    Preț: 37488 lei
  • 18% Microbiome Analysis: Methods and Protocols
    Preț: 135552 lei
  • 18% Computational Methods in Protein Evolution
    Preț: 109722 lei
  • 18% Electrophoretic Separation of Proteins: Methods and Protocols
    Preț: 136778 lei
  • 18% Microbial Metabolomics: Methods and Protocols
    Preț: 109261 lei
  • 18% SNAREs: Methods and Protocols
    Preț: 93855 lei

Preț: 63395 lei

Preț vechi: 74583 lei
-15% Nou

Puncte Express: 951
Preț estimativ în valută:
12134€ • 12646$ • 10101£

Carte tipărită la comandă

Livrare economică 06-20 ianuarie 25

 Adaugă în coș

Wish list
Gift list
Am citit!
Adaugă în listă
Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9780896039889
ISBN-10: 0896039889
Pagini: 360
Ilustrații: XX, 360 p. 71 illus.
Dimensiuni: 155 x 235 x 29 mm
Greutate: 0.76 kg
Ediția:2004
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Research

Cuprins

BAC Library Construction.- Construction of Small Genome BAC Library for Functional and Genomic Applications.- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging.- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions.- Purification of BAC DNA.- Hybridization-Based Selection of BAC Clones.- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction.- BAC Mapping Using Fluorescence In Situ Hybridization.- High-Throughput BAC Fingerprinting.- BAC End Sequencing.- Radiation Hybrid Mapping With BAC Ends.- Shotgun Library Construction for DNA Sequencing.- Rolling Circle Amplification for Sequencing Templates.- Transposon-Mediated Sequencing.- Pyrosequencing.- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions.- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions.- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers.- BAC Finishing Strategies.- Using the TIGR Assembler in Shotgun Sequencing Projects.- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers.- Optimized Multiplex Polymerase Chain Reaction.- Assembly of DNA Sequencing Data.- Sequence Finishing.- Quality Assessment of Finished BAC Sequences.

Textul de pe ultima copertă

The ability to transfer and maintain DNA both within and between species is an essential skill in biotechnology and medicine. In Bacterial Artificial Chromosomes, expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1: Library Construction, Physical Mapping, and Sequencing presents readily reproducible techniques for library construction, physical mapping, and sequencing. The laboratory protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms.
Comprehensive and cutting-edge, the two volumes of Bacterial Artificial Chromosomes provide a superlative collection of highly productive protocols that will prove useful to many bioscientists, including genome sequencers, geneticists, molecular biologists, and biochemists studying the structure and function of specific genomes.

Caracteristici

Includes supplementary material: sn.pub/extras