Cantitate/Preț
Produs

Bacterial Pathogenesis: Methods and Protocols: Methods in Molecular Biology, cartea 1535

Editat de Pontus Nordenfelt, Mattias Collin
en Limba Engleză Paperback – 7 iul 2018
This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (2) 83526 lei  38-44 zile
  Springer Us – 2 iun 2024 83526 lei  38-44 zile
  Springer – 7 iul 2018 87447 lei  6-8 săpt.
Hardback (2) 94053 lei  6-8 săpt.
  Springer – 2 dec 2016 94053 lei  6-8 săpt.
  Springer Us – iun 2023 136609 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 87447 lei

Preț vechi: 106643 lei
-18% Nou

Puncte Express: 1312

Preț estimativ în valută:
16737 17397$ 13864£

Carte tipărită la comandă

Livrare economică 05-19 februarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781493982684
ISBN-10: 1493982680
Ilustrații: XI, 357 p. 71 illus., 48 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.64 kg
Ediția:Softcover reprint of the original 1st ed. 2017
Editura: Springer
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Protein-Based Strategies to Identify and Isolate Bacterial Virulence Factors.- Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics.- Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein-Metabolite Interactions in Bacteria.- Identifying Bacterial Immune Evasion Proteins using Phage Display.- Competition for Iron between Host and Pathogen: A Structural Case Study on Helicobacter pylori.- Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori.- Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities.- Reconstructing the Ancestral Relationships between Bacterial Pathogen Genomes.- Making Fluorescent Streptococci and Enterococci for Live Imaging.- Computer Vision-Based Image Analysis of Bacteria.- Assessing Vacuolar Escape of Listeria Monocytogenes.- Immobilization Techniques of Bacteria for Live Super-Resolution Imaging using Structured Illumination Microscopy.- Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures.- Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes.- Antibody Guided Molecular Imaging of Infective Endocarditis Infection.- The Zebrafish as a Model for Human Bacterial Infections.- Determining Platelet Activation and Aggregation in Response to Bacteria.- Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin and Perforin.- In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci.- Murine Mycobacterium marinum Infection as a Model for Tuberculosis.- Generating and Purifying Fab Fragments from Human and Mouse IgG using the Bacterial Enzymes IdeS, SpeB and Kgp.- Measuring Antibody Orientation at the Bacterial Surface.- Towards Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases.

Textul de pe ultima copertă

This volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains keynotes and implementation advice from the experts Includes supplementary material: sn.pub/extras