Cantitate/Preț
Produs

Bacterial Regulatory RNA: Methods and Protocols: Methods in Molecular Biology, cartea 905

Editat de Kenneth C. Keiler
en Limba Engleză Hardback – 28 iun 2012
The discovery of wide-spread RNA-based regulation in bacteria has led to new evaluations of the importance of bacterial regulatory RNA in every aspect of bacterial physiology. In Bacteria Regulatory RNA: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study bacterial regulatory RNA. These include methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction, and interactions between regulatory RNAs and proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 Authoritative and practical, Bacteria Regulatory RNA: Methods and Protocols seeks to aid scientists in the further study of bacterial regulatory RNA.
Citește tot Restrânge

Toate formatele și edițiile

Toate formatele și edițiile Preț Express
Paperback (1) 87192 lei  6-8 săpt.
  Humana Press Inc. – 23 aug 2016 87192 lei  6-8 săpt.
Hardback (1) 70432 lei  6-8 săpt.
  Humana Press Inc. – 28 iun 2012 70432 lei  6-8 săpt.

Din seria Methods in Molecular Biology

Preț: 70432 lei

Preț vechi: 74138 lei
-5% Nou

Puncte Express: 1056

Preț estimativ în valută:
13480 14220$ 11233£

Carte tipărită la comandă

Livrare economică 02-16 ianuarie 25

Preluare comenzi: 021 569.72.76

Specificații

ISBN-13: 9781617799488
ISBN-10: 1617799483
Pagini: 285
Ilustrații: XI, 333 p.
Dimensiuni: 178 x 254 x 25 mm
Greutate: 0.82 kg
Ediția:2012
Editura: Humana Press Inc.
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

 Bioinformatic Discovery of Bacterial Regulatory RNAs using SIPH.-How to Identify CRISPRs in Sequencing Data.-A Strategy for Identifying Non-coding RNAs using Whole-genome Tiling Arrays.-Genetic Screens to Identify Bacterial sRNA Regulators.-Competition Assays Using Barcoded Deletion Strains to Gain Insight Into Small RNA Function.-Use of Semi-quantitative Northern Blot Analysis to Determine Relative Quantities of Bacterial CRISPR Transcripts.-RNA Visualization in Bacteria by Fluorescence in Situ Hybridization.-RNA Structure Prediction: An Overview of Methods.-Crystallization of RNA-Protein Complexes: From Synthesis and Purification of Individual Components to Crystals.-Analysis of RNA Folding and Ligand Binding by Conventional and High-Throughput Calorimetry.-Use of Aptamer Tagging to Identify in vivo Protein Binding Partners of Small Regulatory RNAs.-Gel Mobility Shift Assays to Detect Protein-RNA Interactions.-RNase Footprinting of Protein Binding Sites on an mRNA Target of Small RNAs.-Computational Identification of sRNA Targets.-Detection of sRNA-mRNA Interactions by Electrophoretic Mobility Shift Assay.-Activity of Small RNAs on the Stability of Targeted mRNAs in vivo.-Native Gel Electrophoresis to Study the Binding and Release of RNA Polymerase by 6S RNA.-Ribosome Purification Approaches for Studying Interactions of Regulatory Proteins and RNAs with the Ribosome.-Analysis of Aminoacyl- and Peptidyl-tRNAs by Gel Electrophoresis.- In vitro trans-translation Assays.

Textul de pe ultima copertă

The discovery of wide-spread RNA-based regulation in bacteria has led to new evaluations of the importance of bacterial regulatory RNA in every aspect of bacterial physiology. In Bacteria Regulatory RNA: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study bacterial regulatory RNA. These include methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction, and interactions between regulatory RNAs and proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
 
 Authoritative and practical, Bacteria Regulatory RNA: Methods and Protocols seeks to aid scientists in the further study of bacterial regulatory RNA.

Caracteristici

Collects vital methods for identification of bacterial regulatory RNAs and characterization of their biochemical properties and activities Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras