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Bifidobacteria: Methods and Protocols: Methods in Molecular Biology, cartea 2278

Editat de Douwe van Sinderen, Marco Ventura
en Limba Engleză Paperback – 4 mar 2022
This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
  Authoritative and cutting-edge,  Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

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Specificații

ISBN-13: 9781071612767
ISBN-10: 107161276X
Pagini: 239
Ilustrații: X, 239 p. 26 illus., 18 illus. in color.
Dimensiuni: 178 x 254 mm
Greutate: 0.45 kg
Ediția:1st ed. 2021
Editura: Springer Us
Colecția Humana
Seria Methods in Molecular Biology

Locul publicării:New York, NY, United States

Cuprins

Methods for the Isolation and Recovery of Bifidobacteria.- Bifidobacterium Transformation.- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria.- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes.- Site-directed Mutagenesis of Bifidobacterium Strains.- Protocol to Select Bifidobacteria from Fecal and Environmental Samples.- Phageome Analysis of Bifidobacteria-Rich Samples.- Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria.- Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides.- Determination of Bifidobacterial Carbohydrate Utilisation Abilities and Associated Metabolic End-Products.- Model for Murine Gut Colonisation by Bifidobacteria.- Identification of Bifidobacteria by the Phosphoketolase Assay.- Determination of Bile Salt Hydrolase Activity in Bifidobacteria.- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.- Metagenomic Analyses of Bifidobacterial Communities.- Resistance of Bifidobacteria toward Antibiotics.- In vitro Assessment of Prebiotic Activity.- Bifidobacterium Genome Assembly and Methylome Analysis using Pacbio SMRT Sequencing.  

 
 
 

Textul de pe ultima copertă

This volume provides current protocols that can be used in various experimental settings involving bifidobacteria. Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  
Authoritative and cutting-edge,  Bifidobacteria: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Caracteristici

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts